PDF(Pubmed)
Abstract：
Despite continuous medical advancements, traumatic brain injury (TBI) remains a leading cause of death and disability worldwide. Consequently, there is a pursuit for biomarkers that allow non-invasive monitoring of patients after cranial trauma, potentially improving clinical management and reducing complications and mortality. Aquaporins (AQPs), which are crucial for transmembrane water transport, may be significant in this context. This study included 48 patients, with 27 having acute (aSDH) and 21 having chronic subdural hematoma (cSDH). Blood plasma samples were collected from the participants at three intervals: the first sample before surgery, the second at 15 h, and the third at 30 h post-surgery. Plasma concentrations of AQP1, AQP2, AQP4, and AQP9 were determined using the sandwich ELISA technique. CT scans were performed on all patients pre- and post-surgery. Correlations between variables were examined using Spearman\'s nonparametric rank correlation coefficient. A strong correlation was found between aquaporin 2 levels and the volume of chronic subdural hematoma and midline shift. However, no significant link was found between aquaporin levels (AQP1, AQP2, AQP4, and AQP9) before and after surgery for acute subdural hematoma, nor for AQP1, AQP4, and AQP9 after surgery for chronic subdural hematoma. In the chronic SDH group, AQP2 plasma concentration negatively correlated with the midline shift measured before surgery (Spearman\'s ρ -0.54; p = 0.017) and positively with hematoma volume change between baseline and 30 h post-surgery (Spearman\'s ρ 0.627; p = 0.007). No statistically significant correlation was found between aquaporin plasma levels and hematoma volume for AQP1, AQP2, AQP4, and AQP9 in patients with acute SDH. There is a correlation between chronic subdural hematoma volume, measured radiologically, and serum AQP2 concentration, highlighting aquaporins\' potential as clinical biomarkers.
摘要：
尽管医学不断进步，创伤性脑损伤（TBI）仍然是全球范围内死亡和残疾的主要原因。因此,人们一直在寻求生物标志物，以允许对颅脑外伤后的患者进行非侵入性监测，有可能改善临床管理，降低并发症和死亡率。水通道蛋白（AQP），这对跨膜水运输至关重要，在这种情况下可能很重要。这项研究包括48名患者，其中27例患有急性（aSDH），21例患有慢性硬膜下血肿（cSDH）。以三个间隔从参与者那里收集血浆样本：手术前的第一个样本，第二个在15小时，第三个在手术后30小时。使用夹心ELISA技术测定AQP1，AQP2，AQP4和AQP9的血浆浓度。对所有患者在手术前后进行CT扫描。使用Spearman的非参数等级相关系数检查变量之间的相关性。水通道蛋白2水平与慢性硬膜下血肿体积和中线移位之间存在很强的相关性。然而,在急性硬膜下血肿手术前后，水通道蛋白水平（AQP1、AQP2、AQP4和AQP9）之间没有发现显著联系，慢性硬膜下血肿术后AQP1，AQP4和AQP9也没有。在慢性SDH组中，AQP2血浆浓度与术前测量的中线移位呈负相关（Spearman'sρ-0.54；p=0.017），与基线和术后30h之间的血肿体积变化呈正相关（Spearman'sρ0.627；p=0.007）。急性SDH患者水通道蛋白血浆AQP1、AQP2、AQP4和AQP9水平与血肿体积无统计学相关性。慢性硬膜下血肿体积之间存在相关性，放射学测量，和血清AQP2浓度，强调水通道蛋白作为临床生物标志物的潜力。
