PDF(Pubmed)
Abstract：
Different developmental genes shape frequent dynamic inter-chromosomal contacts with rDNA units in human and Drosophila cells. In the course of differentiation, changes in these contacts occur, coupled with changes in the expression of hundreds of rDNA-contacting genes. The data suggest a possible role of nucleoli in the global regulation of gene expression. However, the mechanism behind the specificity of these inter-chromosomal contacts, which are rebuilt in every cell cycle, is not yet known. Here, we describe the strong association of rDNA-contacting genes with numerous long intergenic non-coding RNAs (lincRNAs) in HEK293T cells and in initial and differentiated K562 cells. We observed that up to 600 different lincRNAs were preferentially co-expressed with multiple overlapping sets of rDNA-contacting developmental genes, and there was a strong correlation between the genomic positions of rDNA-contacting genes and lincRNA mappings. These two findings suggest that lincRNAs might guide the corresponding developmental genes toward rDNA clusters. We conclude that the inter-chromosomal interactions of rDNA-contacting genes with nucleoli might be guided by lincRNAs, which might physically link particular genomic regions with rDNA clusters.
摘要：
不同的发育基因塑造了人类和果蝇细胞中与rDNA单元频繁的动态染色体间接触。在分化过程中，这些接触发生变化，加上数百个rDNA接触基因的表达变化。数据表明，核仁在基因表达的整体调控中可能发挥作用。然而,这些染色体间接触的特异性背后的机制，在每个细胞周期中重建，还不知道。这里,我们描述了在HEK293T细胞以及初始和分化的K562细胞中，rDNA接触基因与许多长基因间非编码RNA（lincRNA）的强关联。我们观察到多达600种不同的lincRNAs优先与多组rDNA接触发育基因重叠共表达。rDNA接触基因的基因组位置和lincRNA映射之间存在很强的相关性。这两个发现表明lincRNAs可能引导相应的发育基因朝向rDNA簇。我们得出结论，rDNA接触基因与核仁的染色体间相互作用可能由lincRNAs指导，这可能将特定的基因组区域与rDNA簇物理连接。
