Abstract：
BACKGROUND: Atypical acinar cell foci (AACF) seen in pancreatic cancer are fatal and have been studied with some causative agents. However, for the first time, the effect of acetylsalicylic acid with nitric oxide (NO-ASA) on AACF was examined in this study. Although NO-ASA has very successful inhibitory effects against some types of cancer, it has not been investigated whether they can exert their inhibition effects on AACFs.
METHODS: For experimental purposes, 21 14-day-old male Wistar albino rats were used. Azaserine (30 mg/kg) was dissolved in 0.9% NaCl solution and injected intraperitoneally (i.p.) into 14 rats, except for the Control group (Cont) rats, for three weeks. Rats that were injected with azaserine once a week for three weeks and those that did not receive treatment were divided into experimental groups. 15 days after the end of the azaserine injection protocol, NO-ASA was applied to azaserine with NO-ASA (Az+NO-ASA) group rats three consecutive times with an interval of 15 days by gavage. At the end of the 5-month period, pancreatic tissue was dissected and weighed. Pancreas preparations prepared from histological sections were examined for AACF burden and analyzed via a video image analyzer. One-way analysis of variance (ANOVA) non-parametric statistical analyses were performed to test whether there was a difference between the averages of the experimental and Control groups.
RESULTS: AACF burden in both groups injected with azaserine was found to be statistically significant in all categories compared to that of the Control group (p < 0.05). The average Calculated Estimated average AACF volume (mm3) values, the Calculated estimated average AACF diameter (μm), the Estimated average number of AACF per unit volume, AACF rate as a % of Calculated Organ Volume were higher in the AzCont group rats than in the Az+NO-ASA group, when compared, and there was an important level statistical difference between the groups (p < 0.05). It was determined that for all parameters AACFs load in Az+NO-ASA group rats were significantly reduced compared to that of AzCont group rats (p < 0.05).
CONCLUSIONS: We observed that, as a result of the NO-ASA application, the experimental AACF focus ratio created by azaserine injection was significantly inhibited. The inhibitory effect of AACFs in Az+NO-ASA group rats may have resulted from the significant and independent chemopreventive and/or chemotherapeutic activity of NO-ASA against exocrine pancreatic AACF foci.
METHODS: For experimental purposes, 21 14-day-old male Wistar albino rats were used. Azaserine (30 mg/kg) was dissolved in 0.9% NaCl solution and injected intraperitoneally (i.p.) into 14 rats, except for the Control group (Cont) rats, for three weeks. Rats that were injected with azaserine once a week for three weeks and those that did not receive treatment were divided into experimental groups. 15 days after the end of the azaserine injection protocol, NO-ASA was applied to azaserine with NO-ASA (Az+NO-ASA) group rats three consecutive times with an interval of 15 days by gavage. At the end of the 5-month period, pancreatic tissue was dissected and weighed. Pancreas preparations prepared from histological sections were examined for AACF burden and analyzed via a video image analyzer. One-way analysis of variance (ANOVA) non-parametric statistical analyses were performed to test whether there was a difference between the averages of the experimental and Control groups.
RESULTS: AACF burden in both groups injected with azaserine was found to be statistically significant in all categories compared to that of the Control group (p < 0.05). The average Calculated Estimated average AACF volume (mm3) values, the Calculated estimated average AACF diameter (μm), the Estimated average number of AACF per unit volume, AACF rate as a % of Calculated Organ Volume were higher in the AzCont group rats than in the Az+NO-ASA group, when compared, and there was an important level statistical difference between the groups (p < 0.05). It was determined that for all parameters AACFs load in Az+NO-ASA group rats were significantly reduced compared to that of AzCont group rats (p < 0.05).
CONCLUSIONS: We observed that, as a result of the NO-ASA application, the experimental AACF focus ratio created by azaserine injection was significantly inhibited. The inhibitory effect of AACFs in Az+NO-ASA group rats may have resulted from the significant and independent chemopreventive and/or chemotherapeutic activity of NO-ASA against exocrine pancreatic AACF foci.
摘要：
背景：在胰腺癌中看到的非典型腺泡细胞灶（AACF）是致命的，并且已经用一些病原体进行了研究。然而,第一次,研究了乙酰水杨酸与一氧化氮（NO-ASA）对AACF的影响。尽管NO-ASA对某些类型的癌症有非常成功的抑制作用，尚未研究它们是否可以对AACF发挥抑制作用。
方法：出于实验目的，使用21只14天大的雄性Wistar白化病大鼠。将Azaserine（30mg/kg）溶解在0.9％NaCl溶液中，并腹膜内（腹膜内）注射到14只大鼠中，除了对照组(Cont)大鼠，三个星期.每周一次注射氮素的大鼠，持续三周，未接受治疗的大鼠分为实验组。Azaserine注射方案结束后15天，将NO-ASA与NO-ASA（AzNO-ASA）组大鼠连续3次，间隔15天，灌胃。在5个月期间结束时，解剖胰腺组织并称重。检查从组织切片制备的胰腺制剂的AACF负荷并通过视频图像分析仪进行分析。进行单向方差分析(ANOVA)非参数统计分析以测试实验组和对照组的平均值之间是否存在差异。
结果：发现与对照组相比，在所有类别中注射氮杂苦素的AACF负荷均具有统计学意义（p<0.05）。计算的平均AACF估计平均体积(mm3)值，计算的平均AACF直径(μm)，每单位体积AACF的估计平均数量，AzCont组大鼠的AACF率占计算器官体积的百分比高于AzNO-ASA组，当比较时，组间存在重要水平统计学差别(p<0.05)。确定对于所有参数,Az+NO-ASA组大鼠的AACF负荷与AzCont组大鼠相比显著降低(p<0.05)。
结论：我们观察到，作为NO-ASA申请的结果，阿扎司林注射液产生的实验性AACF焦点比受到显著抑制。AzNO-ASA组大鼠中AACF的抑制作用可能是由于NO-ASA对外分泌胰腺AACF病灶具有显着且独立的化学预防和/或化疗活性。
方法：出于实验目的，使用21只14天大的雄性Wistar白化病大鼠。将Azaserine（30mg/kg）溶解在0.9％NaCl溶液中，并腹膜内（腹膜内）注射到14只大鼠中，除了对照组(Cont)大鼠，三个星期.每周一次注射氮素的大鼠，持续三周，未接受治疗的大鼠分为实验组。Azaserine注射方案结束后15天，将NO-ASA与NO-ASA（AzNO-ASA）组大鼠连续3次，间隔15天，灌胃。在5个月期间结束时，解剖胰腺组织并称重。检查从组织切片制备的胰腺制剂的AACF负荷并通过视频图像分析仪进行分析。进行单向方差分析(ANOVA)非参数统计分析以测试实验组和对照组的平均值之间是否存在差异。
结果：发现与对照组相比，在所有类别中注射氮杂苦素的AACF负荷均具有统计学意义（p<0.05）。计算的平均AACF估计平均体积(mm3)值，计算的平均AACF直径(μm)，每单位体积AACF的估计平均数量，AzCont组大鼠的AACF率占计算器官体积的百分比高于AzNO-ASA组，当比较时，组间存在重要水平统计学差别(p<0.05)。确定对于所有参数,Az+NO-ASA组大鼠的AACF负荷与AzCont组大鼠相比显著降低(p<0.05)。
结论：我们观察到，作为NO-ASA申请的结果，阿扎司林注射液产生的实验性AACF焦点比受到显著抑制。AzNO-ASA组大鼠中AACF的抑制作用可能是由于NO-ASA对外分泌胰腺AACF病灶具有显着且独立的化学预防和/或化疗活性。
