PDF(Pubmed)
Abstract：
The mining industry strives to reduce its water footprint by recycling water in ore processing. This leads to build-up of ions, flotation chemicals and microbial biomass, which may affect the process. The Boliden Kevitsa mine in Northern Finland is exposed to seasonal change and recycles up to 90% of the process water. We studied the variation in size, composition and putative functions of microbial communities in summer and winter in the ore processing plant. The raw water, Cu and Ni thickener overflow waters had statistically significantly higher bacterial numbers in winter compared to summer, and specific summer and winter communities were identified. Metagenomic analysis indicated that Cu and Hg resistance genes, sulphate/thiosulphate, molybdate, iron(III) and zinc ABC transporters, nitrate reduction, denitrification, thiosulphate oxidation and methylotrophy were more common in winter than in summer. Raw water drawn from the nearby river did not affect the microbial communities in the process samples, indicating that the microbial communities and metabolic capacities develop within the process over time in response to the conditions in the processing plant, water chemistry, used chemicals, ore properties and seasonal variation. We propose that the microbial community structures are unique to the Boliden Kevitsa mine and processing plant.
摘要：
采矿业努力通过在矿石加工中回收水来减少其水足迹。这导致离子的积聚，浮选化学品和微生物生物质，这可能会影响过程。芬兰北部的BolidenKevitsa矿暴露于季节性变化，可回收高达90％的工艺用水。我们研究了大小的变化，矿石加工厂夏季和冬季微生物群落的组成和假定功能。原水，与夏季相比，冬季的Cu和Ni增稠剂溢流水具有统计学上明显更高的细菌数量，并确定了特定的夏季和冬季社区。宏基因组分析表明，铜和汞的抗性基因，硫酸盐/硫代硫酸盐，钼酸盐,铁（III）和锌ABC转运蛋白，硝酸盐还原，反硝化,硫代硫酸盐氧化和甲基营养在冬季比夏季更常见。从附近河流抽取的原水不影响过程样品中的微生物群落，表明微生物群落和代谢能力在过程中随着时间的推移而发展，以响应加工厂的条件，水化学,用过的化学品，矿石性质和季节变化。我们认为微生物群落结构是BolidenKevitsa矿和加工厂独有的。
