PDF(Pubmed)
Abstract：
Zearalenone (ZEN) is a prevalent mycotoxin found in grains and grain-derived products, inducing adverse health effects in both animals and humans. The in-field application of microorganisms to degrade and detoxify ZEN is a promising strategy to enhance the safety of food and feed. In this study, we investigated the potential of three actinobacterial strains to degrade and detoxify ZEN in vitro and in planta on wheat ears. The residual ZEN concentration and toxicity in the samples were analysed with UHPLC-MS/MS and a bioluminescence BLYES assay, respectively. Streptomyces rimosus subsp. rimosus LMG19352 could completely degrade and detoxify 5 mg/L ZEN in LB broth within 24 h, along with significant reductions in ZEN concentration both in a minimal medium (MM) and on wheat ears. Additionally, it was the only strain that showed a significant colonisation of these ears. Rhodococcus sp. R25614 exhibited partial but significant degradation in LB broth and MM, whereas Streptomyces sp. LMG16995 degraded and detoxified ZEN in LB broth after 72 h by 39% and 33%, respectively. Although all three actinobacterial strains demonstrated the metabolic capability to degrade and detoxify ZEN in vitro, only S. rimosus subsp. rimosus LMG19352 showed promising potential to mitigate ZEN in planta. This distinction underscores the importance of incorporating in planta screening assays for assessing the potential of mycotoxin-biotransforming microorganisms as biocontrol agents.
摘要：
玉米赤霉烯酮（ZEN）是一种普遍存在于谷物和谷物衍生产品中的霉菌毒素，对动物和人类都有不利的健康影响。现场应用微生物降解和解毒ZEN是提高食品和饲料安全性的有前途的策略。在这项研究中,我们研究了三种放线菌菌株在小麦穗上的体外和植物中降解和解毒ZEN的潜力。用UHPLC-MS/MS和生物发光BLYES测定法分析样品中的残余ZEN浓度和毒性,分别。条状链霉菌亚种。rimosusLMG19352可以在24小时内完全降解和解毒LB肉汤中的5mg/LZEN，以及在基本培养基（MM）和小麦穗中ZEN浓度的显着降低。此外,这是唯一表现出这些耳朵明显定殖的菌株。红球菌。R25614在LB肉汤和MM中表现出部分但显著的降解，而链霉菌sp。LMG16995在LB肉汤中在72小时后降解和脱毒的ZEN分别为39％和33％，分别。尽管所有三个放线菌菌株都证明了在体外降解和解毒ZEN的代谢能力，只有S.rimosus亚科。rimosusLMG19352显示出减轻植物中ZEN的有希望的潜力。这种区别强调了纳入植物筛选测定法以评估霉菌毒素生物转化微生物作为生物防治剂的潜力的重要性。
