PDF(Pubmed)
Abstract：
The growing demand for phycobiliproteins from microalgae generates a significant volume of by-products, such as extraction cakes. These cakes are enriched with products of interest for the cosmetics market, namely free fatty acids, particularly polyunsaturated (PUFA). In this work, two cakes, one of spirulina and one of Porphyridium cruentum, were valorized using innovative natural hydrophobic deep eutectic solvents (NaDES) based on alkanediols. The most promising NaDES, as determined by physicochemical properties and screening, are mixtures of alkanediols and fatty acids. These include the mixtures of 1,3-propanediol and octanoic acid (1:5, mol/mol) and 1,3-propanediol and octanoic and decanoic acid (1:3:1, mol/mol). Two extractive processes were implemented: ultrasound-assisted extraction and an innovative mechanical process involving dual asymmetric centrifugation. The second process resulted in the production of extracts significantly enriched in PUFA, ranging from 65 to 220 mg/g dry matter with the two cakes. The extracts and NaDES demonstrated good safety with respect to epidermal keratinocyte viability (>80% at 200 µg/mL). The study of their impact on commensal and pathogenic cutaneous bacteria demonstrated significant effects on the viability of Staphylococcus aureus and Staphylococcus epidermidis (>50% decrease at 200 µg/mL) while preserving Corynebacterium xerosis and Cutibacterium acnes. These results highlight the potential of valorizing these co-products using alkanediol-based NaDES, in a strategy combining an active vector (NaDES) and a growth regulator extract, for the management of cutaneous dysbiosis involving staphylococci.
摘要：
对来自微藻的藻胆蛋白的不断增长的需求产生了大量的副产物，如提取蛋糕。这些蛋糕富含化妆品市场感兴趣的产品，即游离脂肪酸，特别是多不饱和（PUFA）。在这项工作中,两个蛋糕,一种螺旋藻和一种卟啉藻，使用基于链烷二醇的创新天然疏水性深共熔溶剂（NaDES）进行了有效的评估。最有希望的纳德斯,根据物理化学性质和筛选确定，是烷二醇和脂肪酸的混合物。这些包括1,3-丙二醇和辛酸(1:5,mol/mol)以及1,3-丙二醇和辛酸和癸酸(1:3:1,mol/mol)的混合物。实施了两个提取过程：超声辅助提取和涉及双重不对称离心的创新机械过程。第二个过程导致产生显著富含PUFA的提取物，范围从65到220毫克/克干物质与两个蛋糕。提取物和NaDES在表皮角质形成细胞活力方面表现出良好的安全性（200μg/mL时>80％）。它们对共生和致病性皮肤细菌的影响的研究表明，对金黄色葡萄球菌和表皮葡萄球菌的生存力有显着影响（在200µg/mL时降低>50％），同时保留干燥棒杆菌和痤疮杆菌。这些结果突出了使用基于烷二醇的NaDES使这些副产物增值的潜力，在结合活性载体（NaDES）和生长调节剂提取物的策略中，用于治疗涉及葡萄球菌的皮肤菌群失调。
