PDF(Pubmed)
Abstract：
UNASSIGNED: This research was to innovate a nanozyme-based therapeutic strategy that combines aggregation-induced emission (AIE) photosensitizers with copper nanozymes. This approach is designed to address the hypoxic conditions often found in bacterial infections and aims to boost the effectiveness of photodynamic therapy (PDT) by ensuring sufficient oxygen supply for reactive oxygen species (ROS) generation.
UNASSIGNED: Our approach involved the synthesis of dihydroxyl triphenyl vinyl pyridine (DHTPY)-Cu@zoledronic acid (ZOL) nanozyme particles. We initially synthesized DHTPY and then combined it with copper nanozymes to form the DHTPY-Cu@ZOL composite. The nanozyme\'s size, morphology, and chemical properties were characterized using various techniques, including dynamic light scattering, transmission electron microscopy, and X-ray photoelectron spectroscopy. We conducted a series of in vitro and in vivo tests to evaluate the photodynamic, antibacterial, and wound-healing properties of the DHTPY-Cu@ZOL nanozymes, including their oxygen-generation capacity, ROS production, and antibacterial efficacy against methicillin-resistant Staphylococcus aureus (MRSA).
UNASSIGNED: The DHTPY-Cu@ZOL exhibited proficient H2O2 scavenging and oxygen generation, crucial for enhancing PDT in oxygen-deprived infection environments. Our in vitro analysis revealed a notable antibacterial effect against MRSA, suggesting the nanozymes\' potential to disrupt bacterial cell membranes. Further, in vivo studies using a diabetic rat model with MRSA-infected wounds showed that DHTPY-Cu@ZOL markedly improved wound healing and reduced bacterial presence, underscoring its efficacy as a non-antibiotic approach for chronic infections.
UNASSIGNED: Our study suggests that DHTPY-Cu@ZOL is a highly promising approach for combating antibiotic-resistant microbial pathogens and biofilms. The biocompatibility and stability of these nanozyme particles, coupled with their improved PDT efficacy position them as a promising candidate for clinical applications.
UNASSIGNED: Our approach involved the synthesis of dihydroxyl triphenyl vinyl pyridine (DHTPY)-Cu@zoledronic acid (ZOL) nanozyme particles. We initially synthesized DHTPY and then combined it with copper nanozymes to form the DHTPY-Cu@ZOL composite. The nanozyme\'s size, morphology, and chemical properties were characterized using various techniques, including dynamic light scattering, transmission electron microscopy, and X-ray photoelectron spectroscopy. We conducted a series of in vitro and in vivo tests to evaluate the photodynamic, antibacterial, and wound-healing properties of the DHTPY-Cu@ZOL nanozymes, including their oxygen-generation capacity, ROS production, and antibacterial efficacy against methicillin-resistant Staphylococcus aureus (MRSA).
UNASSIGNED: The DHTPY-Cu@ZOL exhibited proficient H2O2 scavenging and oxygen generation, crucial for enhancing PDT in oxygen-deprived infection environments. Our in vitro analysis revealed a notable antibacterial effect against MRSA, suggesting the nanozymes\' potential to disrupt bacterial cell membranes. Further, in vivo studies using a diabetic rat model with MRSA-infected wounds showed that DHTPY-Cu@ZOL markedly improved wound healing and reduced bacterial presence, underscoring its efficacy as a non-antibiotic approach for chronic infections.
UNASSIGNED: Our study suggests that DHTPY-Cu@ZOL is a highly promising approach for combating antibiotic-resistant microbial pathogens and biofilms. The biocompatibility and stability of these nanozyme particles, coupled with their improved PDT efficacy position them as a promising candidate for clinical applications.
摘要：
■这项研究旨在创新一种基于纳米酶的治疗策略，该策略将聚集诱导的发射（AIE）光敏剂与铜纳米酶相结合。这种方法旨在解决细菌感染中经常出现的缺氧状况，旨在通过确保足够的氧气供应来产生活性氧（ROS）来提高光动力疗法（PDT）的有效性。
■我们的方法涉及二羟基三苯基乙烯基吡啶（DHTPY）-Cu@唑来膦酸（ZOL）纳米酶颗粒的合成。我们最初合成了DHTPY，然后将其与铜纳米酶结合形成DHTPY-Cu@ZOL复合材料。纳米酶的大小，形态学,使用各种技术表征化学性质，包括动态光散射，透射电子显微镜,和X射线光电子能谱。我们进行了一系列的体外和体内测试，以评估光动力，抗菌，DHTPY-Cu@ZOL纳米酶的伤口愈合特性，包括它们的氧气产生能力，ROS生产，和对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌效果。
■DHTPY-Cu@ZOL表现出熟练的H2O2清除和氧气生成，在缺氧感染环境中增强PDT至关重要。我们的体外分析显示对MRSA有显著的抗菌作用，表明纳米酶有可能破坏细菌细胞膜。Further,使用MRSA感染伤口的糖尿病大鼠模型进行的体内研究表明，DHTPY-Cu@ZOL显着改善了伤口愈合并减少了细菌的存在，强调其作为慢性感染的非抗生素方法的功效。
■我们的研究表明，DHTPY-Cu@ZOL是一种非常有前途的对抗抗生素抗性微生物病原体和生物膜的方法。这些纳米酶颗粒的生物相容性和稳定性，加上其改善的PDT疗效使他们成为临床应用的有希望的候选人。
■我们的方法涉及二羟基三苯基乙烯基吡啶（DHTPY）-Cu@唑来膦酸（ZOL）纳米酶颗粒的合成。我们最初合成了DHTPY，然后将其与铜纳米酶结合形成DHTPY-Cu@ZOL复合材料。纳米酶的大小，形态学,使用各种技术表征化学性质，包括动态光散射，透射电子显微镜,和X射线光电子能谱。我们进行了一系列的体外和体内测试，以评估光动力，抗菌，DHTPY-Cu@ZOL纳米酶的伤口愈合特性，包括它们的氧气产生能力，ROS生产，和对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌效果。
■DHTPY-Cu@ZOL表现出熟练的H2O2清除和氧气生成，在缺氧感染环境中增强PDT至关重要。我们的体外分析显示对MRSA有显著的抗菌作用，表明纳米酶有可能破坏细菌细胞膜。Further,使用MRSA感染伤口的糖尿病大鼠模型进行的体内研究表明，DHTPY-Cu@ZOL显着改善了伤口愈合并减少了细菌的存在，强调其作为慢性感染的非抗生素方法的功效。
■我们的研究表明，DHTPY-Cu@ZOL是一种非常有前途的对抗抗生素抗性微生物病原体和生物膜的方法。这些纳米酶颗粒的生物相容性和稳定性，加上其改善的PDT疗效使他们成为临床应用的有希望的候选人。
