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Abstract：
Background: The vascular endothelial growth factor (VEGF) polymorphism is associated with preeclampsia since its abnormal expression plays an important role in vasculogenesis in placenta formation. Thus, this study is aimed at analyzing the association between VEGF +936C/T polymorphism and the risk of preeclampsia. Methods: To assess the causal relationship, a hospital-based cross-sectional analytical study was carried out among 204 Myanmar pregnant women during the period of January 2018-September 2020. For data collection, a pretested, structured questionnaire was used. Blood samples were collected after obtaining consent, and then we studied the extracted gene by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The Statistical Package for Social Sciences version 18.0 was used for data management and analysis. Results: The genotype CT variant among preeclamptic women was more than that of non-preeclamptic women (26.5% vs. 18.6%), but not significant (p = 0.180). The risk of preeclampsia among women with CT genotypes was 1.57 times higher than that of women with CC genotypes (OR (95%CI) = 1.57 (0.81, 3.06), p = 0.180). The minor allele frequency of the T allele was 15.2% in preeclamptic women and 9.3% in normal pregnant women. The risk of preeclampsia among T allele carriers is 1.49 times (95%CI = 0.80, 2.77) more than that of C allele carriers (p = 0.211). Among the preeclamptic pregnant women, the frequency of the CT genotype was 26.3% in the severe preeclamptic group and 26.9% in the mild preeclamptic group, while the frequency of the T allele was 13.2% and 13.5%, respectively. The frequency of either CT genotype or T allele was more or less the same in both groups, and there was no association between VEGF C/T polymorphism and the severity of preeclampsia. After logistic regression analysis on VEGF genotype and clinical parameters such as age, maternal body mass index (BMI), and neonatal birth weight, the risk of preeclampsia was 2.1 times higher in pregnant women with CT genotype compared to CC genotype (adjusted OR, 2.1; 95% CI, 0.9-4.5, p value -0.057). Conclusion: There was no significant association between VEGF +936C/T polymorphism (rs3025039) and preeclampsia among Myanmar pregnant women. However, the findings of this study highlighted that individuals carrying either the CT genotype or the T allele are at a heightened risk of developing preeclampsia. Furthermore, it suggests a potential impact of the gene on the occurrence of preeclampsia, yet the data lacks sufficient evidence to establish statistical significance.
摘要：
背景：血管内皮生长因子（VEGF）多态性与子痫前期有关，因为其异常表达在胎盘形成的血管生成中起重要作用。因此，本研究旨在分析VEGF+936C/T多态性与先兆子痫风险的相关性.方法：评估因果关系，在2018年1月至2020年9月期间,对204名缅甸孕妇进行了基于医院的横断面分析研究.对于数据收集，一个预先测试，采用结构化问卷。在征得同意后采集血样，然后我们用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）研究了提取的基因。社会科学统计软件包18.0版用于数据管理和分析。结果：先兆子痫妇女的基因型CT变异高于非先兆子痫妇女（26.5％vs.18.6%），但不显著(p=0.180)。具有CT基因型的女性发生子痫前期的风险是具有CC基因型的女性的1.57倍（OR（95CI）=1.57（0.81，3.06），p=0.180）。T等位基因的次要等位基因频率在先兆子痫妇女中为15.2％，在正常孕妇中为9.3％。T等位基因携带者的子痫前期风险是C等位基因携带者的1.49倍（95CI=0.80，2.77）（p=0.211）。在先兆子痫孕妇中，CT基因型频率在重度子痫前期组为26.3%,在轻度子痫前期组为26.9%,而T等位基因的频率为13.2%和13.5%，分别。两组CT基因型或T等位基因的频率或多或少相同，VEGFC/T多态性与子痫前期的严重程度无相关性。对VEGF基因型和年龄等临床参数进行logistic回归分析,孕妇体重指数（BMI），和新生儿出生体重，与CC基因型相比，CT基因型的孕妇先兆子痫的风险高2.1倍（调整后的OR，2.1；95%CI，0.9-4.5，p值-0.057）。结论:VEGF+936C/T多态性(rs3025039)与缅甸孕妇子痫前期无明显相关性。然而,这项研究的结果强调,携带CT基因型或T等位基因的个体患先兆子痫的风险增加.此外，这表明该基因对先兆子痫的发生有潜在影响，然而，数据缺乏足够的证据来确定统计意义。
