PDF(Pubmed)
Abstract：
RNA-based therapeutics have emerged as a promising approach for the treatment of various diseases, including cancer, genetic disorders, and infectious diseases. However, the delivery of RNA molecules into target cells has been a major challenge due to their susceptibility to degradation and inefficient cellular uptake. To overcome these hurdles, DNA-based nano technology offers an unprecedented opportunity as a potential delivery platform for RNA therapeutics. Due to its excellent characteristics such as programmability and biocompatibility, these DNA-based nanostructures, composed of DNA molecules assembled into precise and programmable structures, have garnered significant attention as ideal building materials for protecting and delivering RNA payloads to the desired cellular destinations. In this review, we highlight the current progress in the design and application of three DNA-based nanostructures: DNA origami, lipid-nanoparticle (LNP) technology related to frame guided assembly (FGA), and DNA hydrogel for the delivery of RNA molecules. Their biomedical applications are briefly discussed and the challenges and future perspectives in this field are also highlighted.
摘要：
基于RNA的疗法已成为治疗各种疾病的有希望的方法，包括癌症,遗传性疾病,和传染病。然而,由于RNA分子易于降解和细胞摄取效率低下,因此将RNA分子递送到靶细胞中一直是主要挑战。为了克服这些障碍，基于DNA的纳米技术作为RNA疗法的潜在递送平台提供了前所未有的机会。由于其优异的特性，如可编程性和生物相容性，这些基于DNA的纳米结构，由组装成精确可编程结构的DNA分子组成，作为保护RNA有效载荷并将其递送到所需细胞目的地的理想建筑材料，已经引起了极大的关注。在这次审查中,我们重点介绍了三种基于DNA的纳米结构的设计和应用的当前进展：DNA折纸，与框架引导组装（FGA）相关的脂质纳米颗粒（LNP）技术，和用于递送RNA分子的DNA水凝胶。简要讨论了它们的生物医学应用，并强调了该领域的挑战和未来前景。
