Abstract：
In diabetic patients with skin injuries, bacterial proliferation, accumulation of reactive oxygen species (ROS) in the tissues, and impaired angiogenesis make wound healing difficult. Therefore, eliminating bacteria, removing ROS, and promoting angiogenesis are necessary for treating acute diabetic wounds. In this study, benefiting from the ability of polyphenols to form a metal-phenolic network (MPN) with metal ions, TA-Eu MPN nanoparticles (TM NPs) were synthesized. The prepared photothermal agent CuS NPs and TM NPs were then loaded onto the supporting base and needle tips of PVA/HA (PH) microneedles, respectively, to obtain PH/CuS/TM microneedles. Antibacterial experiments showed that microneedles loaded with CuS NPs could remove bacteria by the photothermal effect. In vitro experiments showed that the microneedles could effectively scavenge ROS, inhibit macrophage polarization to the M1 type, and induce polarization to the M2 type as well as have the ability to promote vascular endothelial cell migration and angiogenesis. Furthermore, in vivo experiments showed that PH/CuS/TM microneedles accelerated wound healing by inhibiting pro-inflammatory cytokines and promoting angiogenesis in a diabetic rat wound model. Therefore, PH/CuS/TM microneedles have efficient antibacterial, ROS scavenging, anti-inflammatory, immunomodulatory, and angiogenic abilities and hold promise as wound dressings for treating acute diabetic wounds.
摘要：
在有皮肤损伤的糖尿病患者中，细菌增殖，活性氧（ROS）在组织中的积累，受损的血管生成使伤口难以愈合。因此,消除细菌，去除ROS，和促进血管生成是治疗急性糖尿病伤口所必需的。在这项研究中,受益于多酚与金属离子形成金属-酚网络（MPN）的能力，合成了TA-EuMPN纳米颗粒(TMNP)。然后将制备的光热剂CuSNPs和TMNPs加载到PVA/HA（PH）微针的支撑基座和针尖上，分别,获得PH/CuS/TM微针。抗菌实验表明，负载CuSNPs的微针可以通过光热效应去除细菌。体外实验表明,微针能够有效清除ROS,抑制巨噬细胞极化为M1型，并诱导极化为M2型，并具有促进血管内皮细胞迁移和血管生成的能力。此外，体内实验表明，PH/CuS/TM微针通过抑制促炎细胞因子和促进糖尿病大鼠伤口模型的血管生成来加速伤口愈合。因此,PH/CuS/TM微针具有高效抗菌，ROS清除，抗炎,免疫调节,和血管生成能力，并有望作为治疗急性糖尿病伤口的伤口敷料。
