PDF(Pubmed)
Abstract：
Cyanobacterium Nostoc commune is a filamentous terrestrial prokaryotic organism widely distributed, which suggest its high adaptive potential to environmental or abiotic stress. Physiological parameters and proteomic analysis were performed in two accession of N. commune with the aim to elucidate the differences of physiological trails between distant geotypes, namely Antarctic (AN) and central European (CE). The result obtained clearly showed that the AN geotype demonstrates elevated levels of total phenols, flavonoids, carotenoids, and phycobiliproteins, indicative of its adaptation to environmental stress as referred by comparison to CE sample. Additionally, we employed LC-MS analysis to investigate the proteomes of N. commune from AN and CE geotypes. In total, 1147 proteins were identified, among which 646 proteins expressed significant (up-regulation) changes in both accessions. In the AN geotype, 83 exclusive proteins were identified compared to 25 in the CE geotype. Functional classification of the significant proteins showed a large fraction involved in photosynthesis, amino acid metabolism, carbohydrate metabolism and protein biosynthesis. Further analysis revealed some defense-related proteins such as, superoxide dismutase (SOD) and glutathione reductase, which are rather explicitly expressed in the AN N. commune. The last two proteins suggest a more stressful condition in AN N. commune. In summary, our findings highlight biochemical processes that safeguard the AN geotype of N. commune from extreme environmental challenges, not recorded in CE accession, probably due to less stressful environment in Europe. This study brings the first ever proteomic analysis of N. commune, emphasizing the need for additional investigations into the climate adaptation of this species with rather plastic genome.
摘要：
蓝藻是一种分布广泛的丝状陆生原核生物,这表明它对环境或非生物胁迫具有很高的适应性。在N.commune的两个登录处进行了生理参数和蛋白质组学分析，目的是阐明遥远地理类型之间生理轨迹的差异，即南极(AN)和中欧(CE)。获得的结果清楚地表明，AN地理型表明总酚水平升高，黄酮类化合物，类胡萝卜素，和藻胆蛋白，通过与CE样品的比较，表明其对环境压力的适应性。此外,我们采用LC-MS分析研究了来自AN和CE地理型的N.commune的蛋白质组。总的来说,鉴定了1147种蛋白质，其中646种蛋白质在两个种质中都表达了显着（上调）变化。在AN几何图形中，与CE地理型中的25个相比,鉴定了83个专有蛋白。重要蛋白质的功能分类显示大部分参与光合作用，氨基酸代谢，碳水化合物代谢和蛋白质生物合成。进一步的分析揭示了一些防御相关的蛋白质，例如，超氧化物歧化酶（SOD）和谷胱甘肽还原酶，这在ANN.公社中相当明确地表达。最后两种蛋白质表明ANN公社的压力更大。总之,我们的发现强调了生化过程，保护N.commune的一个地理类型免受极端环境挑战，没有记录在CE加入中，可能是由于欧洲压力较小的环境。这项研究带来了有史以来第一次N.commune的蛋白质组学分析，强调需要对具有相当可塑性基因组的该物种的气候适应性进行进一步研究。
