PDF(Pubmed)
Abstract：
Fluorescence nanoscopy, also known as super-resolution microscopy, has transcended the conventional resolution barriers and enabled visualization of biological samples at nanometric resolutions. A series of super-resolution techniques have been developed and applied to investigate the molecular distribution, organization, and interactions in blood cells, as well as the underlying mechanisms of blood-cell-associated diseases. In this review, we provide an overview of various fluorescence nanoscopy technologies, outlining their current development stage and the challenges they are facing in terms of functionality and practicality. We specifically explore how these innovations have propelled forward the analysis of thrombocytes (platelets), erythrocytes (red blood cells) and leukocytes (white blood cells), shedding light on the nanoscale arrangement of subcellular components and molecular interactions. We spotlight novel biomarkers uncovered by fluorescence nanoscopy for disease diagnosis, such as thrombocytopathies, malignancies, and infectious diseases. Furthermore, we discuss the technological hurdles and chart out prospective avenues for future research directions. This review aims to underscore the significant contributions of fluorescence nanoscopy to the field of blood cell analysis and disease diagnosis, poised to revolutionize our approach to exploring, understanding, and managing disease at the molecular level.
摘要：
荧光纳米显微镜,也被称为超分辨率显微镜，已经超越了传统的分辨率障碍，并使纳米分辨率的生物样品的可视化。已经开发了一系列超分辨率技术并将其用于研究分子分布，组织,和血细胞中的相互作用，以及血细胞相关疾病的潜在机制。在这次审查中,我们提供了各种荧光纳米技术的概述，概述了他们目前的发展阶段以及他们在功能和实用性方面面临的挑战。我们特别探索这些创新如何推动血小板(血小板)的分析，红细胞（红细胞）和白细胞（白细胞），亚细胞组分和分子相互作用的纳米级排列上的光。我们聚焦用于疾病诊断的荧光纳米显微镜发现的新型生物标志物，比如血小板病变,恶性肿瘤,和传染病。此外，我们讨论了技术障碍，并为未来的研究方向绘制了前景。这篇综述旨在强调荧光纳米显微镜对血细胞分析和疾病诊断领域的重要贡献。准备彻底改变我们的探索方法，理解,在分子水平上管理疾病。
