Abstract：
Linear IgA bullous dermatosis (LABD) and dermatitis herpetiformis (DH) represent the major subtypes of IgA mediated autoimmune bullous disorders. We sought to understand the disease etiology by using serum proteomics. We assessed 92 organ damage biomarkers in LAB, DH, and healthy controls using the Olink high-throughput proteomics. The positive proteomic serum biomarkers were used to correlate with clinical features and HLA type. Targeted proteomic analysis of IgA deposition bullous disorders vs. controls showed elevated biomarkers. Further clustering and enrichment analyses identified distinct clusters between LABD and DH, highlighting the involvement of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. Comparative analysis revealed biomarkers with distinction between LABD and DH and validated in the skin lesion. Finally, qualitative correlation analysis with DEPs suggested six biomarkers (NBN, NCF2, CAPG, FES, BID, and PXN) have better prognosis in DH patients. These findings provide potential biomarkers to differentiate the disease subtype of IgA deposition bullous disease.
摘要：
线性IgA大疱性皮肤病（LABD）和疱疹样皮炎（DH）代表IgA介导的自身免疫性大疱性疾病的主要亚型。我们试图通过使用血清蛋白质组学来了解疾病的病因。我们评估了LAB中的92种器官损伤生物标志物，DH,和健康对照使用Olink高通量蛋白质组学。阳性蛋白质组血清生物标志物用于与临床特征和HLA类型相关。IgA沉积大疱性疾病的靶向蛋白质组学分析与对照显示生物标志物升高。进一步的聚类和富集分析确定了LABD和DH之间的不同簇，强调烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶的参与。比较分析揭示了在LABD和DH之间具有区别的生物标志物,并且在皮肤损伤中得到验证。最后,与DEP的定性相关分析表明有六种生物标志物(NBN，NCF2,CAPG,FES,BID,和PXN)在DH患者中有较好的预后。这些发现提供了潜在的生物标志物来区分IgA沉积大疱性疾病的疾病亚型。
