Abstract：
Disulfiram (DSF) metabolites exhibit antitumor properties when bound to Cu2+. This combination also promotes the generation of reactive oxygen species (ROS), ultimately leading to tumor cell death. In this study, CuO2 served as a carrier for DSF, forming a dual-drug delivery system with Cu2+ and DSF encapsulated in polydopamine (PDA). In the final delivery system, CuO2 (DSF-CuO2@PDA) was hydrolyzed at the tumor site, releasing both Cu2+ and H2O2. Cu2+ reacts with DSF metabolites to form Bis(diethyldithiocarbamate)-Cu (CuET), which triggers a Fenton-like reaction that generates ROS. Chemotherapy and chemodynamic therapy exhibited significant tumor-suppressive capabilities, with an inhibition rate of 61 %. In addition, the DSF-CuO2@PDA complex demonstrated superlative tumor-targeting ability and biocompatibility.
摘要：
双硫仑（DSF）代谢物与Cu2结合时表现出抗肿瘤特性。这种组合还促进了活性氧（ROS）的产生，最终导致肿瘤细胞死亡。在这项研究中,CuO2作为DSF的载体，形成Cu2+和DSF封装在聚多巴胺(PDA)中的双给药系统。在最终交付系统中，CuO2（DSF-CuO2@PDA）在肿瘤部位水解，同时释放Cu2+和H2O2。Cu2+与DSF代谢物反应形成双(二乙基二硫代氨基甲酸酯)-Cu(CuET),引发类似Fenton的反应，产生ROS。化疗和化学动力学治疗表现出显著的肿瘤抑制能力，抑制率为61%。此外,DSF-CuO2@PDA复合物表现出最高级的肿瘤靶向能力和生物相容性。
