PDF(Pubmed)
Abstract：
BACKGROUND: In Addis Ababa, Ethiopia, open ditches along innner roads in residential areas serve to convey domestic wastewater and rainwater away from residences. Contamination of drinking water by wastewater through faulty distribution lines could expose households to waterborne illnesses. This prompted the study to assess the microbiological safety of wastewater and drinking water in Addis Ababa, identify the pathogens therein, and determine their antibiotic resistance patterns.
UNASSIGNED: O1, mainly Hikojima serotype, was isolated from 23 wastewater and 16 drinking water samples. Similarly, 19 wastewater and 10 drinking water samples yielded Escherichia coli O157:H7. V. cholerae O1 were 100% resistant to the penicillins (Amoxacillin and Ampicillin), and 51-82% were resistant to the cephalosporins. About 44% of the V. cholerae O1 isolates in this study were Extended Spectrum Beta-Lactamase (ESBL) producers. Moreover, 26% were resistant to Meropenem. Peperacillin/Tazobactam was the only effective β-lactam antibiotic against V. cholerae O1. V. cholerae O1 isolates showed 37 different patterns of multiple resistance ranging from a minimum of three to a maximum of ten antimicrobials. Of the E. coli O157:H7 isolates, 71% were ESBL producers. About 96% were resistant to Ampicillin. Amikacin and Gentamicin were very effective against E. coli O157:H7 isolates. The isolates from wastewater and drinking water showed multiple antibiotic resistance against three to eight antibiotic drugs.
CONCLUSIONS: Open ditches for wastewater conveyance along innner roads in residence areas and underground faulty municipal water distribution lines could be possible sources for V. cholerae O1 and E. coli O157:H7 infections to surrounding households and for dissemination of multiple drug resistance in humans and, potentially, the environment.
UNASSIGNED: O1, mainly Hikojima serotype, was isolated from 23 wastewater and 16 drinking water samples. Similarly, 19 wastewater and 10 drinking water samples yielded Escherichia coli O157:H7. V. cholerae O1 were 100% resistant to the penicillins (Amoxacillin and Ampicillin), and 51-82% were resistant to the cephalosporins. About 44% of the V. cholerae O1 isolates in this study were Extended Spectrum Beta-Lactamase (ESBL) producers. Moreover, 26% were resistant to Meropenem. Peperacillin/Tazobactam was the only effective β-lactam antibiotic against V. cholerae O1. V. cholerae O1 isolates showed 37 different patterns of multiple resistance ranging from a minimum of three to a maximum of ten antimicrobials. Of the E. coli O157:H7 isolates, 71% were ESBL producers. About 96% were resistant to Ampicillin. Amikacin and Gentamicin were very effective against E. coli O157:H7 isolates. The isolates from wastewater and drinking water showed multiple antibiotic resistance against three to eight antibiotic drugs.
CONCLUSIONS: Open ditches for wastewater conveyance along innner roads in residence areas and underground faulty municipal water distribution lines could be possible sources for V. cholerae O1 and E. coli O157:H7 infections to surrounding households and for dissemination of multiple drug resistance in humans and, potentially, the environment.
摘要：
背景：在亚的斯亚贝巴，埃塞俄比亚,住宅区内恩纳道路上的明沟可将生活污水和雨水从住宅中运走。废水通过错误的分配管线污染饮用水可能会使家庭遭受水传播疾病的侵害。这促使该研究评估亚的斯亚贝巴的废水和饮用水的微生物安全性，确定其中的病原体，并确定它们的抗生素抗性模式。
■O1，主要是平岛血清型，从23个废水和16个饮用水样品中分离出来。同样，19个废水和10个饮用水样品产生大肠杆菌O157:H7。霍乱弧菌O1对青霉素（阿莫西林和氨苄西林）具有100％抗性，51-82％对头孢菌素耐药。在这项研究中，约有44％的霍乱弧菌O1分离物是超广谱β-内酰胺酶（ESBL）生产者。此外，26%对美罗培南耐药。哌拉西林/他唑巴坦是唯一有效的抗霍乱弧菌O1的β-内酰胺抗生素。霍乱弧菌O1分离株显示出37种不同的多重耐药模式，范围从最少3种到最多10种抗生素。在大肠杆菌O157:H7分离株中，71%是ESBL生产者。约96%对氨苄西林有抗性。阿米卡星和庆大霉素对大肠杆菌O157:H7分离株非常有效。来自废水和饮用水的分离株对三到八种抗生素药物显示出多种抗生素抗性。
结论：沿着居住区的innner道路和地下有问题的市政配水线输送废水的开放式沟渠可能是霍乱弧菌O1和大肠杆菌O157：H7感染到周围家庭的可能来源，并在人类中传播多种耐药性，潜在的,环境。
■O1，主要是平岛血清型，从23个废水和16个饮用水样品中分离出来。同样，19个废水和10个饮用水样品产生大肠杆菌O157:H7。霍乱弧菌O1对青霉素（阿莫西林和氨苄西林）具有100％抗性，51-82％对头孢菌素耐药。在这项研究中，约有44％的霍乱弧菌O1分离物是超广谱β-内酰胺酶（ESBL）生产者。此外，26%对美罗培南耐药。哌拉西林/他唑巴坦是唯一有效的抗霍乱弧菌O1的β-内酰胺抗生素。霍乱弧菌O1分离株显示出37种不同的多重耐药模式，范围从最少3种到最多10种抗生素。在大肠杆菌O157:H7分离株中，71%是ESBL生产者。约96%对氨苄西林有抗性。阿米卡星和庆大霉素对大肠杆菌O157:H7分离株非常有效。来自废水和饮用水的分离株对三到八种抗生素药物显示出多种抗生素抗性。
结论：沿着居住区的innner道路和地下有问题的市政配水线输送废水的开放式沟渠可能是霍乱弧菌O1和大肠杆菌O157：H7感染到周围家庭的可能来源，并在人类中传播多种耐药性，潜在的,环境。
