Abstract：
Chloramphenicol (CAP) poses a threat to human health due to its toxicity and bioaccumulation, and it is very important to measure it accurately and sensitively. This work explored a host-guest recognition strategy to mediate dual aggregation-induced electrochemiluminescence (AIECL) of 1,1,2,2-tetrakis(4-(pyridin-4-yl) phenyl)-ethene (TPPE) for ratio detection of CAP, in which, cucurbit[8]uril (CB[8]) served as host to assemble guest TPPE. The resulting supramolecular complex CB[8]-TPPE exhibited excellent dual-AIECL-emission with signal strength approximately four times that of TPPE aggregates and black hole quencher-1 (BHQ1) could efficiently quench dual-AIECL signal. CB[8]-TPPE coupled dual-function quencher BHQ1 and high-efficiency DNA reactor to achieve ultra-sensitive detection of CAP, exhibiting a linearity range of 10 fmol·L-1-100 nmol·L-1 and limit of detection of 1.81 fmol·L-1. CB[8]-TPPE provides a novel way to improve the dual-emission of TPE derivatives and sets up a promising platform for CAP detection, demonstrating a good practical application potential.
摘要：
氯霉素（CAP）由于其毒性和生物蓄积性，对人类健康构成威胁，准确、灵敏地测量是非常重要的。这项工作探索了一种宿主-客体识别策略，以介导1,1,2,2-四（4-（吡啶-4-基）苯基）-乙烯（TPPE）的双重聚集诱导的电化学发光（AIECL），用于CAP的比率检测，其中，葫芦[8]uril（CB[8]）作为组装客人TPPE的主机。所得的超分子复合物CB[8]-TPPE表现出优异的双AIECL发射，信号强度约为TPPE聚集体的四倍，黑洞猝灭剂1（BHQ1）可以有效地猝灭双AIECL信号。CB[8]-TPPE偶联双功能猝灭剂BHQ1和高效DNA反应器实现对CAP的超灵敏检测,线性范围为10fmol·L-1-100nmol·L-1，检出限为1.81fmol·L-1。CB[8]-TPPE为改善TPE衍生物的双发射提供了一种新的途径，为CAP检测建立了一个有前途的平台，展示了良好的实际应用潜力。
