Abstract：
The Papaya ringspot virus (PRSV)-resistant genetically modified (GM) papaya \'Huanong No.1\' has been certified as safe for consumption and widely planted in China for about 18 years. To protect consumers\' rights and facilitate government supervision and monitoring, it is necessary to establish a simple, rapid, and specific detection method for \'Huanong No.1\'. Herein, we developed a platform based on recombinase polymerase amplification (RPA) coupled with CRISPR-Cas12a for the detection of \'Huanong No.1\'. The RPA-CRISPR-Cas12a platform was found to have high specificity, with amplification signals only present in \'Huanong No.1\'. Additionally, the platform was highly sensitive, with a limit of detection (LOD) of approximately 20 copies. The detection process was fast and could be completed in less than 1 h. This novel platform enables the rapid on-site visualization detection of \'Huanong No.1\', eliminating dependence on laboratory conditions and specialized instruments, and can serve as a technical reference for the rapid detection of other GM plants.
摘要：
抗木瓜环斑病毒（PRSV）的转基因（GM）木瓜“HuanongNo.1”已被证明可以安全食用，并在中国广泛种植了约18年。为了保护消费者权利，促进政府监督和监测，有必要建立一个简单的，快速,以及“华农一号”的具体检测方法。在这里，我们开发了一个基于重组酶聚合酶扩增（RPA）和CRISPR-Cas12a的平台，用于检测\'HuanongNo.1\'。发现RPA-CRISPR-Cas12a平台具有高特异性，放大信号仅存在于“华农一号”中。此外,平台高度敏感，检测限（LOD）约为20份。检测过程快速，可在不到1小时内完成。该新颖的平台可实现“华农一号”的现场快速可视化检测，消除对实验室条件和专用仪器的依赖，并可作为其他转基因植物快速检测的技术参考。
