Abstract：
In this study, the pathogenicity of local Beauveria bassiana strains was elucidated using molecular and metabolomics methodologies. Molecular verification of the B. bassiana-specific chitinase gene was achieved via phylogenetic analysis of the Bbchit1 region. Subsequent metabolomic analyses employing UPLC-Q-TOF-MS revealed a different number of non-volatile metabolite profiles among the six B. bassiana strains. Bb6 produced the most non-volatile compounds (17) out of a total of 18, followed by Bb15 (16) and Bb12 (15). Similarly, Bb5, Bb8, and Bb21, three non-virulent B. bassiana strains, produced 13, 14, and 14 metabolites, respectively. But unique secondary metabolites like bassianolide and beauvericin, pivotal for virulence and mite management, were exclusively found in the virulent strains (Bb6, Bb12, and Bb15) of B. bassiana. The distinctive non-volatile metabolomic profiles of these strains underscore their pathogenicity against Tetranychus truncatus, suggesting their promise in bio-control applications.
摘要：
在这项研究中,使用分子和代谢组学方法阐明了当地白僵菌菌株的致病性。通过Bbchit1区域的系统发育分析，实现了对球孢芽孢杆菌特异性几丁质酶基因的分子验证。采用UPLC-Q-TOF-MS的随后的代谢组学分析揭示了在6种白孢芽孢杆菌菌株中不同数量的非挥发性代谢物谱。在总共18种化合物中,Bb6产生最多的非挥发性化合物(17种),其次是Bb15(16)和Bb12(15)。同样，Bb5，Bb8和Bb21，三个非毒力的白僵菌菌株，产生13、14和14种代谢物，分别。但是独特的次生代谢产物，如硅藻内酯和白维菌素，毒力和螨管理的关键，仅在球孢芽孢杆菌的强毒株（Bb6，Bb12和Bb15）中发现。这些菌株独特的非挥发性代谢组学特征强调了它们对干Tetranychus的致病性，表明他们在生物防治应用中的前景。
