PDF(Pubmed)
Abstract：
The aim of this paper was to evaluate the degree of mycological air contamination and determine the taxonomic diversity of airborne fungi residing in the air of 20 different animal facilities in a zoological garden. The concentrations of fungi in the zoological garden were measured using a MAS-100 air sampler. The collected microorganisms were identified using the combination of molecular and morphological methods. The fungal concentration ranged from 50 to 3.65 × 104 CFU/m3 during the whole study. The quantitative analysis of the fungal aerosol showed that the obtained concentration values were lower than the recommended permissible limits (5 × 104 CFU/m3 for fungi). Environmental factors, including temperature and relative humidity, exerted a varying effect on the presence and concentration of isolated fungi. Relative humidity was shown to correlate positively with the concentration of fungal spores in the air of the facilities studied (rho = 0.57, p < 0.0021). In parallel, no significant correlation was established between temperature and total fungal concentration (rho = - 0.1, p < 0.2263). A total of 112 fungal strains belonging to 50 species and 10 genera were isolated. Penicillium was the dominant genera, including 58.9% of total fungal strains, followed by Aspergillus 25.89%, Cladosporium 3.57%, Talaromyces 3.57%, Mucor 1.78%, Schizophyllum 1.78%, Syncephalastrum 0.89%, Alternaria 0.89%, Absidia 0.89%, and Cunninghamella 0.89%. Our preliminary studies provide basic information about the fungal concentrations, as well as their biodiversity in zoological garden. Further studies are needed to generate additional data from long-term sampling in order to increase our understanding of airborne fungal composition in the zoological garden.
摘要：
本文的目的是评估真菌空气污染的程度，并确定在动物园中20个不同动物设施的空气中存在的空气中真菌的分类多样性。使用MAS-100空气采样器测量了动物园中真菌的浓度。使用分子和形态学方法的组合鉴定收集的微生物。在整个研究过程中，真菌浓度范围为50至3.65×104CFU/m3。真菌气溶胶的定量分析表明，获得的浓度值低于推荐的允许限值（真菌为5×104CFU/m3）。环境因素,包括温度和相对湿度，对分离真菌的存在和浓度产生了不同的影响。相对湿度显示与所研究设施空气中真菌孢子的浓度呈正相关（rho=0.57，p<0.0021）。并行,在温度和总真菌浓度之间没有建立显着相关性（rho=-0.1，p<0.2263）。共分离出50种10属112株真菌。青霉属是优势属,包括总真菌菌株的58.9%，其次是曲霉25.89%,枝孢霉3.57%,Talaromyces3.57%,Mucor1.78%,裂子菌1.78%,Synheadastrum0.89%,链格孢菌0.89%,Absidia0.89%,和Cunninghamella0.89%。我们的初步研究提供了有关真菌浓度的基本信息，以及它们在动物园中的生物多样性。需要进一步的研究来从长期采样中产生更多数据，以增加我们对动物园中空气传播的真菌成分的了解。
