Abstract：
Pathogen proliferation and virulence depend on available nutrients, and these vary when the pathogen moves from outside of the host cell (extracellular) to the inside of the host cell (intracellular). Nuclear Magnetic Resonance (NMR) is a versatile analytical method, which lends itself for metabolic studies. In this chapter, we describe how 1H NMR can be combined with a cellular infection model to study the metabolic crosstalk between a bacterial pathogen and its host both in the extracellular and intracellular compartments. Central carbon metabolism is highlighted by using glucose labeled with the stable isotope 13C.
摘要：
病原体的增殖和毒力取决于可用的营养物质，当病原体从宿主细胞外部（细胞外）移动到宿主细胞内部（细胞内）时，这些变化。核磁共振（NMR）是一种通用的分析方法，这有助于代谢研究。在这一章中,我们描述了如何将1HNMR与细胞感染模型相结合，以研究细菌病原体与其宿主在细胞外和细胞内区室中的代谢串扰。通过使用用稳定同位素13C标记的葡萄糖来突出中心碳代谢。
