Abstract：
Human immunodeficiency virus (HIV) infection is still a global public health issue, and the development of an effective prophylactic vaccine inducing potent neutralizing antibodies remains a significant challenge. This study aims to explore the inflammation-related proteins associated with the neutralizing antibodies induced by the DNA/rTV vaccine. In this study, we employed the Olink chip to analyze the inflammation-related proteins in plasma in healthy individuals receiving HIV candidate vaccine (DNA priming and recombinant vaccinia virus rTV boosting) and compared the differences between neutralizing antibody-positive (nab + ) and -negative(nab-) groups. We identified 25 differentially expressed factors and conducted enrichment and correlation analysis on them. Our results revealed that significant expression differences in artemin (ARTN) and C-C motif chemokine ligand 23 (CCL23) between nab+ and -nab- groups. Notably, the expression of CCL23 was negatively corelated to the ID50 of neutralizing antibodies and the intensity of the CD4+ T cell responses. This study enriches our understanding of the immune picture induced by the DNA/rTV vaccine, and provides insights for future HIV vaccine development.
摘要：
人类免疫缺陷病毒（HIV）感染仍然是一个全球性的公共卫生问题，和开发有效的预防性疫苗诱导强效中和抗体仍然是一个重大的挑战。本研究旨在探讨与DNA/rTV疫苗诱导的中和抗体相关的炎症相关蛋白。在这项研究中,我们使用Olink芯片分析了接受HIV候选疫苗(DNA引发和重组牛痘病毒rTV增强)的健康个体血浆中的炎症相关蛋白,并比较了中和抗体阳性(nab+)和阴性(nab-)组之间的差异.我们确定了25个差异表达因子，并对其进行了富集和相关性分析。我们的结果表明，nab和-nab-组之间artemin（ARTN）和C-C基序趋化因子配体23（CCL23）的表达存在显着差异。值得注意的是,CCL23的表达与中和抗体的ID50和CD4+T细胞应答的强度呈负相关.本研究丰富了我们对DNA/rTV疫苗诱导的免疫图景的认识,并为未来的HIV疫苗开发提供见解。
