RESULTS: 30 adult male Wistar rats were divided into 5 groups with 6 animals in each group: Control; Thymoquinone (TQ); Corn oil; Chronic Sleep restriction (CSR); and CSR + TQ. After 30 days, behavioral analysis, antioxidant, lipid profile, glucose level, liver and kidney function test, neurotransmitters, neuropeptides, and mRNA expression in in vivo studies were also assessed and pharmacokinetic and docking were done for thymoquinone. Thymoquinone has also shown good binding affinity to the target proteins. CSR has induced oxidative stress in the discrete brain regions and plasma. Current study has shown many evidences that sleep restriction has altered the neurobehavioral, antioxidant status, lipid profile, neurotransmitters, neuropeptide levels, and feeding behavior which damage the Orexin-leptin system which regulates the sleep and feeding that leads to metabolic dysfunction.
CONCLUSIONS: The potentiality of Thymoquinone was revealed in in silico studies, and its action in in vivo studies has proved its effectiveness. The study concludes that Thymoquinone has exhibited its effect by diminishing the metabolic dysfunction by its neuroprotective, antioxidant, and hypolipidemic properties.
结果:将30只成年雄性Wistar大鼠分为5组,每组6只动物:对照组;胸醌(TQ);玉米油;慢性睡眠限制(CSR);和CSR+TQ。30天后,行为分析,抗氧化剂,血脂谱,葡萄糖水平,肝肾功能检查,神经递质,神经肽,还评估了体内研究中的mRNA表达,并对百里香醌进行了药代动力学和对接。胸醌还显示了对靶蛋白的良好结合亲和力。CSR在离散的大脑区域和血浆中诱导了氧化应激。目前的研究表明,许多证据表明睡眠限制改变了神经行为,抗氧化状态,血脂谱,神经递质,神经肽水平,和摄食行为,破坏调节睡眠和摄食的食欲素-瘦素系统,导致代谢功能障碍。
结论:在计算机研究中揭示了胸腺醌的潜力,其在体内的作用研究已经证明了其有效性。该研究得出结论,胸腺醌通过其神经保护作用减少代谢功能障碍,抗氧化剂,和降血脂特性。