RESULTS: Acinetobacter baumannii isolates were collected between 2023 and 2024 from diverse clinical samples. Antimicrobial resistance testing followed standardized protocols, with β-lactamase-encoding genes detected via PCR and sequencing. Investigation into ISAba1 upstream of blaOXA-carbapenemase and blaADC genes was also conducted. Genetic diversity was assessed through ERIC-PCR. Among the 78 A. baumannii, widespread resistance to multiple antimicrobials was evident. Various acquired β-lactamase-encoding genes (blaOXA-23,-24,-58,-143, blaVIM, and blaNDM) were detected. Furthermore, this is the first report of blaVIM-2 in A. baumannii isolates harboring either the blaOXA-23-like or the blaOXA-143 gene in Brazil. Molecular typing revealed a high genetic heterogeneity among the isolates, and multi-clonal dissemination.
CONCLUSIONS: The accumulation of genetic resistance determinants underscores the necessity for stringent infection control measures and robust antimicrobial stewardship programs to curb multidrug-resistant strains.
结果:A.鲍曼不动杆菌分离株在2023年至2024年间从不同的临床样本中收集。抗菌素耐药性测试遵循标准化方案,通过PCR和测序检测β-内酰胺酶编码基因。还对blaOXA-碳青霉烯酶和blaADC基因上游的ISAba1进行了调查。通过ERIC-PCR评估遗传多样性。在78个鲍曼不动杆菌中,对多种抗菌药物的广泛耐药是显而易见的。各种获得的β-内酰胺酶编码基因(blaOXA-23,-24,-58,-143,blaVIM,和blaNDM)被检测到。此外,这是blaVIM-2在巴西携带blaOXA-23-样或blaOXA-143基因的鲍曼不动杆菌分离株中的首次报道。分子分型揭示了分离株之间的高度遗传异质性,和多克隆传播。
结论:遗传抗性决定因素的积累强调了严格的感染控制措施和强有力的抗菌药物管理计划以遏制多药耐药菌株的必要性。