Introduction Salt is an essential component of the Indian diet. Edible salt contains fluoride, and its concentration varies depending on the source and manufacturing process. This study aimed to assess the fluoride concentration of commonly available varieties of edible salts in India. Methodology This in vitro study was conducted with four different types of edible salts viz. iodized salt, rock salt, pink salt, and black salt. Two brands of each salt available in the market were procured. Fluoride estimation was conducted using the sodium 2-(parasulphophenylazo-)- 1,8-dihydroxy-3,6-naphthalene disulphonate (SPADNS) method using Fluoride High-Range Checker® HC (Hanna Equipments India Pvt. Ltd., Mumbai, India) with HI739-26 reagent. The reaction between fluoride and the reagent forms a colorless complex in the sample. The concentration is then determined from the color produced, measured in parts per million (ppm). Results Iodized salt showed the least fluoride content (12.5 ± 7.5 ppm), while it was highest for black salt (77.5 ± 19.9 ppm). There was a statistically significant difference between the study groups (p=0.009). Rock salt also showed high amounts of fluoride (40.8 ± 52.4 ppm). Conclusion Fluoride content in different types of edible salt varied, though well within the prescribed limits. This calls for nutritional labelling of fluoride to help the consumer make informed choices.

OBJECTIVE: To compare the accuracy of robotic and dynamic navigation systems in assisting zygomatic implant (ZI) using an in vitro model experiment.
METHODS: Preoperative cone-beam computed tomography (CBCT) images of patients who underwent ZI treatment between 2011 and 2023 were collected from local databases. Corresponding three-dimensional resin models were printed and assigned to two groups: the robotic and dynamic navigation system groups. Following preoperative plans, ZIs were placed in the models with the assistance of either a robotic or dynamic navigation system. Deviations in the in vitro navigation surgery were measured and compared between the groups.
RESULTS: A total of 110 ZIs were placed in 56 models, with 55 ZIs in each group. No significant differences were observed in entry and angle deviations between the groups (p>0.05). However, the exit deviation in the robotic system group (2.39±1.24 mm) was larger than that in the dynamic navigation group (1.83±1.25 mm) (p<0.05). On the exit side, the Z-axis deviation in the robotic group (left: -0.28±1.43 mm, right: -0.21±1.30 mm) was smaller than that in the dynamic navigation group (left: 0.76±1.11 mm, right: 0.85±1.52 mm) (p<0.05), while no significant differences were found in X- and Y-axis deviations (p>0.05).
CONCLUSIONS: Compared with the dynamic navigation system, the robotic system can effectively prevent ZI overextension. However, its accuracy on the exit side is slightly lower than that of the dynamic navigation system.
CONCLUSIONS: This preliminary in vitro study showed that the accuracy of the robotic system was slightly inferior to that of the dynamic navigation system in terms of exit deviation when used in ZI placement. Further clinical studies are required to confirm these findings.

Airborne pathogens represent a topic of scientific relevance, especially considering the recent COVID-19 pandemic. Air pollution, and particulate matter (PM) in particular, has been proposed as a possible risk factor for the onset and spread of pathogen-driven respiratory diseases. Regarding SARS-CoV-2 infection, exposure to fine PM (PM2.5, particles with an aerodynamic diameter < 2.5 μm) has been associated with increased incidence of the COVID-19 disease. To provide useful insights into the mechanisms through which PM might be involved in infection, we exposed human lung cells (A549) to PM2.5 and SARS-CoV-2, to evaluate the toxicological properties and the molecular pathways activated when airborne particles are combined with viral particles. Winter PM2.5 was collected in a metropolitan urban area and its physico-chemical composition was analyzed. A549 cells were exposed to SARS-CoV-2 concomitantly or after pre-treatment with PM2.5. Inflammation, oxidative stress and xenobiotic metabolism were the main pathways investigated. Results showed that after 72 h of exposure PM2.5 significantly increased the expression of the angiotensin-converting enzyme 2 (ACE2) receptor, which is one of the keys used by the virus to infect host cells. We also analyzed the endosomal route in the process of internalization, by studying the expression of RAB5 and RAB7. The results show that in cells pre-activated with PM and then exposed to SARS-CoV-2, RAB5 expression is significantly increased. The activation of the inflammatory process was then studied. Our findings show an increase of pro-inflammatory markers (NF-kB and IL-8) in cells pre-activated with PM for 72 h and subsequently exposed to the virus for a further 24 h, further demonstrating that the interaction between PM and SARS-CoV-2 determines the severity of the inflammatory responses in lung epithelial cells. In conclusion, the study provides mechanistic biological evidence of PM contribution to the onset and progression of viral respiratory diseases in exposed populations.

This study aimed to investigate the accuracy of implants placed by clinicians with varying dental implant experience using dynamic navigation (DN) and static guidance (SG). Sixty identical custom-made drillable maxillary models were fabricated, missing the right central incisor (#8) and left first molar (#14) with simulated gingival tissue. Models planned with a dynamic navigation system and guided surgery software were randomly allocated to an experienced clinician, an inexperienced clinician, and a nonexperienced clinician. The accuracy of implant placement was evaluated by overlaying the preoperative virtual surgical plan with the postoperative Cone Beam Computed Tomography (CBCT) scan. Deviations between the placed and planned implants were quantified using a mathematical algorithm. Independent-sample t-tests revealed significant differences (p < 0.001) in angular deviation but not in coronal and apical deviations when comparing dynamic navigation with static guidance for all three clinicians. One-way ANOVA and Tukey post-hoc test found no significant differences between clinicians of varying dental implant experience in dynamic navigation and static guidance groups. The study concluded that the level of dental implant experience and surgical site do not significantly impact the accuracy of implant placement when either dynamic navigation or static guidance is used. Dynamic navigation produced less angular deviation in comparison with static guidance. This finding suggests dynamic navigation could benefit clinical settings, particularly for less experienced clinicians.

BACKGROUND: Sutures are essential components of wound closure in oral surgery, and the mechanical properties of suture materials play a crucial role in determining surgical outcomes. Understanding the tensile strengths of various suture materials is vital for selecting the most appropriate material for specific clinical applications.
OBJECTIVE: This study aimed to assess the tensile strength of suture materials commonly used in oral surgery through an in vitro tensile strength study.
METHODS: A total of 192 samples of six commonly used suture materials (polyglycolic acid (PGA), polyglactin 910 (PGLA), polylactic acid (PLA), polydioxanone (PDO), silk, and nylon) were subjected to tensile strength testing using a universal testing machine. Descriptive statistics were used to summarize the tensile strength of each suture material. A comparative analysis was conducted using appropriate statistical tests to identify any significant differences in the tensile strength among the different materials.
RESULTS: Significant variability in tensile strength was observed among the suture materials in newtons (N). PGLA exhibited the highest mean tensile strength (38.7 N), followed closely by PDO (37.1 N), whereas silk displayed the lowest tensile strength (32.8 N). Comparative analysis revealed significant differences in the tensile strength among the materials (p < 0.001).
CONCLUSIONS: This study provides valuable insights into the mechanical properties of the suture materials commonly used in oral surgery. These findings underscore the importance of considering tensile strength when selecting suture materials for specific clinical scenarios, thereby optimizing wound closure techniques and improving patient outcomes.

BACKGROUND: Biodentine, a calcium silicate-based material, is known for its biocompatibility and ability to promote dentin regeneration. With their unique branching structure, polyamidoamine (PAMAM) dendrimers have shown promise in facilitating biomimetic remineralization processes.
OBJECTIVE: This study investigates the synergistic effects of combining PAMAM with Biodentine on root dentin remineralization, aiming to develop a novel bioactive compound that offers superior protective and regenerative properties.
METHODS: The following predictions were made: (1) In a cyclic artificial saliva/acid regimen, among the test groups, the combination of Biodentine and PAMAM would cause the most root dentin remineralization (2). Biodentine alone would increase Ca and P concentrations, neutralize acid, and promote root dentin remineralization (3). PAMAM, on the other hand, can remineralize the demineralized root dentin.
RESULTS: Minimal mineral regeneration was accomplished in demineralized root dentin when treated with Biodentine or PAMAM alone. Root dentin remineralization was most pronounced when Biodentine and PAMAM were used together, and the hardness of demineralized root dentin was raised to an equivalent level to that of healthy root dentin.
CONCLUSIONS: The study demonstrated the exceptional ability of PAMAM + Biodentine to promote root dentin remineralization. In an acid-challenging environment, PAMAM + Biodentine promoted full and efficient root dentin remineralization. Restorations made using innovative PAMAM + Biodentine technology show promise in remineralizing and protecting tooth structures.

Numerous factors, including exposure to harmful substances, drinking too much alcohol, contracting certain hepatitis serotypes, and using specific medicines, contribute to the development of liver illnesses. Lipid peroxidation and other forms of oxidative stress are the main mechanisms by which hepatotoxic substances harm liver cells. Pathological changes in the liver include a rise in the levels of blood serum, a decrease in antioxidant enzymes, as well as the formation of free radical radicals. It is necessary to find pharmaceutical alternatives to treat liver diseases to increase their efficacy and decrease their toxicity. For the development of new therapeutic medications, a greater knowledge of primary mechanisms is required. In order to mimic human liver diseases, animal models are developed. Animal models have been used for several decades to study the pathogenesis of liver disorders and related toxicities. For many years, animal models have been utilized to investigate the pathophysiology of liver illness and associated toxicity. The animal models are created to imitate human hepatic disorders. This review enlisted numerous hepatic damage in vitro and in vivo models using various toxicants, their probable biochemical pathways and numerous metabolic pathways via oxidative stressors, different serum biomarkers enzymes are discussed, which will help to identify the most accurate and suitable model to test any plant preparations to check and evaluate their hepatoprotective properties.

The availability of metamizole varies greatly around the world. There are countries such as the USA, UK, or Australia where the use of metamizole is completely forbidden, and there are also countries where this drug is available only on prescription (e.g., Greece, Italy, Spain, etc.) and those in which it is sold OTC-over the counter (e.g., most Asian and South American countries). Metamizole, as a drug with a strong analgesic effect, is used as an alternative to other non-steroidal anti-inflammatory drugs, alone or in combination with opioid drugs. Risedronate sodium is a third-generation bisphosphonate commonly used in orthopaedic and metabolic diseases of the musculoskeletal system, including hypercalcemia, postmenopausal osteoporosis, Paget\'s disease, etc. The aim of this study was to check whether there were any pharmacological interactions between metamizole and risedronate sodium in in vitro studies. Cell viability was assessed using the MTT method, the number of apoptotic cells was assessed using the labelling TUNEL method, and the cell cycle assessment was performed with a flow cytometer and propidium iodide. This was a pilot study, which is why only two cancer cell lines were tested: D-17 of canine osteosarcoma and U-2 OS of human osteosarcoma. Exposure of the canine osteosarcoma cell line to a combination of risedronate sodium (100 µg/mL) and metamizole (50, 5, and 0.5 µg/mL) resulted in the complete abolition of the cytoprotective activity of metamizole. In the human osteosarcoma cell line, the cytotoxic effect of risedronate sodium was entirely eliminated in the presence of 50 µg/mL of metamizole. The cytoprotective and anti-apoptotic effect of metamizole in combination with risedronate sodium in the tested human and canine osteosarcoma cell lines indicates an urgent need for further in vivo studies to confirm or disprove the potential dose-dependent undesirable effect of such a therapy.

OBJECTIVE: Orthodontists are often asked to remove fixed retainers before patients undergo magnetic resonance imaging (MRI). The present in vitro study was designed to analyze the heating and bonding efficacy of stainless steel multibraided fixed retainers after 1.5- and 3-tesla (T) MRI.
METHODS: A total of 180 human mandibular incisors were used to create 45 specimens of four teeth each, divided into nine groups. Handmade multibraided fixed retainers of three different sizes, defined by the diameter of the initial wire used (0.008″, 0.010″ and 0.012″), were tested. Three groups underwent MRI at 1.5 T, another three groups underwent MRI at 3 T and the last three groups did not undergo MRI. Temperature was assessed before and after MRI. Shear bond strength (SBS) and adhesive remnant index (ARI) were assessed after MRI for all groups. Data were statistically analyzed (p < 0.05).
RESULTS: After 1.5 T exposure, no significant temperature increase from T0 to T1 was observed in any of the groups (p > 0.05). Regarding the 3 T groups, a significant difference from T0 to T1 was found for all the groups (p < 0.05). Temperature changes were not clinically relevant, as they were less than 1 °C for all groups except for group 3 (ΔT0-T1: 1.18 ± 0.3 °C) and group 6 (ΔT0-T1: 1.12 ± 0.37 °C). Furthermore, there were no significant differences between the temperature variations associated with different wire diameters (p > 0.05).
CONCLUSIONS: No significant changes in SBS or ARI were found (p > 0.05).
CONCLUSIONS: Since overheating was irrelevant and adhesion values did not change, the tested devices were concluded to be safe for MRI examinations at 1.5 T and 3 T.

UNASSIGNED: White spot lesions occur when the pathogenic bacteria have broken through the enamel layer. White spot lesions (WSLs) can be treated using a complex approach. The most crucial step is to stop demineralization and biofilm formation and use assorted strategies for remineralization of lesions, thinning, microabrasion, erosion infiltration, adhesive composite resin restorations, and the bonded facets.
UNASSIGNED: To evaluate and compare the fluoride, calcium, and phosphorus ion release, remineralizing efficacy, and microhardness of zwitterionic material, self-assembling peptide, and bioactive glass (BGA) incorporated with MI Varnish.
UNASSIGNED: The original study was conducted on 60 extracted premolars; the sample size calculated was 10 per group. All samples were divided into four groups-group A, MI Varnish (control), group B, MI Varnish + zwitterionic material, group C, MI Varnish + self-assembling peptide, and group D, MI Varnish + BGA. All these groups were further evaluated and compared for fluoride, calcium, and phorphorus ion release, remineralizing efficacy, and surface microhardness (SMH).
UNASSIGNED: Zwitterionic material, when incorporated with MI Varnish showed high fluoride and calcium ion release and high remineralizing efficacy under polarized light microscopy (PLM). BGA, when incorporated with MI Varnish showed high phosphorus ion release and higher values in the evaluation of SMH, followed by zwitterionic material and self-assembling peptide.
UNASSIGNED: MI varnish alone had remineralizing properties of WSLs, but when novel materials like zwitterionic ion, self-assembling peptide, and BGA are incorporated, its efficacy increases. Among all zwitterionic ions showed superior results for fluoride and calcium ion release and remineralization and BGA for phosphorus ion release and SMH.
UNASSIGNED: Patil SV, Gugwad SC, Devendrappa SN, et al. Comparative Evaluation of Zwitterionic Material, Self-assembling Peptide, and Bioactive Glass Incorporated with MI Varnish for Fluoride, Calcium, and Phosphorus Ion Release, Enamel Remineralization, and Microhardness. Int J Clin Pediatr Dent 2024;17(S-1):S37-S42.