Abstract：
Clinostomum spp. are common parasites of piscivorous birds. Metacercaria are typically observed in the muscles or just under the skin of fish and rarely amphibians. We describe an unusually severe case of Clinostomum marginatum infection in an adult female green tree frog (Hyla cinerea) from Georgia (USA). The frog was found in November 2015 with a high number (>250) of widely disseminated, raised, subcutaneous nodules. The frog died in December. At necropsy, it was emaciated, and the skin was covered in raised uniform, tan-green, subcutaneous, ∼2-3 mm diameter nodules. Each nodule contained 1-3 C. marginatum metacercariae. Microscopically, high numbers of trematodes were within subcutaneous tissues and in coelomic and oral cavities, lung, liver, kidney, ovary, orbit and calvarium. Small to large numbers of lymphocytes and melanomacrophages were in connective tissues and epidermis. A 732 bp region of COI was 98.8-99.8% similar to numerous sequences of C. marginatum and, phylogenetically it grouped with these C. marginatum sequences. The ITS-1 region was 100% similar to a C. marginatum sample from a great egret (Ardea alba) from Mississippi. This report represents a novel finding of severe trematodiasis in a free-ranging amphibian with C. marginatum infection.
摘要：
Clinostomumspp。是食肉鸟的常见寄生虫。通常在肌肉中或在鱼的皮肤下观察到囊虫，很少有两栖动物。我们描述了来自佐治亚州（美国）的成年雌性绿树蛙（Hylacinerea）的异常严重的Clinodomum边缘感染病例。青蛙于2015年11月被发现，大量（>250）广泛传播，举起,皮下结节。青蛙在12月死亡。尸检时,它消瘦了，皮肤上覆盖着凸起的制服，棕绿色,皮下,直径2-3毫米的结节。每个结节包含1-3个C。边缘cer。微观上,大量的吸虫在皮下组织内以及在体腔和口腔中，肺，肝脏,肾,子房,轨道和颅骨。结缔组织和表皮中存在少量至大量的淋巴细胞和黑素滋养细胞。COI的732bp区域与许多C.marginatum序列相似，为98.8-99.8％，它在系统发育上与这些C.marginatum序列分组。ITS-1区域与来自密西西比州的大白鹭（Ardeaalba）的C.marginatum样本100％相似。该报告代表了在患有C.marginatum感染的自由放养两栖动物中严重吸虫病的新发现。
