Abstract：
In this study, a bioprocessing strategy was designed to valorize ultra-filtered spent sulfite liquor (UF-SSL) without prior detoxification steps as well as using it purely as a carbon source supplement to defined or complex media. Hence, a minimal medium for the bioconversion of UF-SSL with Corynebacterium glutamicum was developed and process robustness and reproducibility were validated. Process quantifiability was ensured by development of a biomass measurement technique for matrices with high water-insoluble solids and verified using elemental balancing. Mechanistic modeling based on Monod equations was used to identify batch kinetics. In a final step, scale-up of the developed process was performed to showcase process maturity towards commercialisation.
摘要：
在这项研究中,设计了一种生物处理策略，以在没有预先解毒步骤的情况下对超滤的废液（UF-SSL）进行增值，并将其纯粹用作定义或复杂培养基的碳源补充。因此，本研究开发了谷氨酸棒杆菌生物转化UF-SSL的基本培养基,并验证了工艺的稳健性和可重复性.通过开发具有高水不溶性固体的基质的生物质测量技术并使用元素平衡进行验证，确保了过程的可量化性。基于Monod方程的力学模型用于识别批量动力学。在最后一步，对开发的工艺进行了放大，以展示工艺向商业化的成熟度。
