{Reference Type}: Journal Article
{Title}: Robust, fully quantifiable and scalable bioprocess utilizing spent sulfite liquor with Corynebacterium glutamicum.
{Author}: Waldschitz D;Neudert MR;Kitzmueller J;Lachmann J;Fonteyne A;Maes K;Bar N;Sinner P;Spadiut O;
{Journal}: Bioresour Technol
{Volume}: 406
{Issue}: 0
{Year}: 2024 Aug 14
{Factor}: 11.889
{DOI}: 10.1016/j.biortech.2024.130967
{Abstract}: In this study, a bioprocessing strategy was designed to valorize ultra-filtered spent sulfite liquor (UF-SSL) without prior detoxification steps as well as using it purely as a carbon source supplement to defined or complex media. Hence, a minimal medium for the bioconversion of UF-SSL with Corynebacterium glutamicum was developed and process robustness and reproducibility were validated. Process quantifiability was ensured by development of a biomass measurement technique for matrices with high water-insoluble solids and verified using elemental balancing. Mechanistic modeling based on Monod equations was used to identify batch kinetics. In a final step, scale-up of the developed process was performed to showcase process maturity towards commercialisation.