%0 Journal Article
%T Robust, fully quantifiable and scalable bioprocess utilizing spent sulfite liquor with Corynebacterium glutamicum.
%A Waldschitz D
%A Neudert MR
%A Kitzmueller J
%A Lachmann J
%A Fonteyne A
%A Maes K
%A Bar N
%A Sinner P
%A Spadiut O
%J Bioresour Technol
%V 406
%N 0
%D 2024 Aug 14
%M 38880268
%F 11.889
%R 10.1016/j.biortech.2024.130967
%X In this study, a bioprocessing strategy was designed to valorize ultra-filtered spent sulfite liquor (UF-SSL) without prior detoxification steps as well as using it purely as a carbon source supplement to defined or complex media. Hence, a minimal medium for the bioconversion of UF-SSL with Corynebacterium glutamicum was developed and process robustness and reproducibility were validated. Process quantifiability was ensured by development of a biomass measurement technique for matrices with high water-insoluble solids and verified using elemental balancing. Mechanistic modeling based on Monod equations was used to identify batch kinetics. In a final step, scale-up of the developed process was performed to showcase process maturity towards commercialisation.