Abstract:
Cysteine (Cys) not only plays an indispensable role in maintaining the redox balance in organisms, but is also an important nutrient in the food industry. Fluorescence-based detection systems have emerged as an effective method to track the locations and concentrations of different species. To achieve efficient monitoring of Cys in both food samples and biological systems, a novel lipid droplet (LD) targeted fluorescent probe (namely NIT-Cys) was constructed for the turn-on detection of Cys, characterized by a large Stokes shift (142 nm), a short response time (<8 min), and a low Cys detection limit (39 nM). Furthermore, the NIT-Cys probe has been successfully used not only to quantify the amounts of Cys in selected food samples, but also to enable the visualization of endogenous Cys in acetaminophen (APAP)-induced drug-induced liver injury cells, zebrafish larvae and mice models. Consequently, the work presented here provides an efficient tool for monitoring Cys.
摘要:
半胱氨酸(Cys)不仅在维持生物体的氧化还原平衡中起着不可或缺的作用,但也是食品工业中的重要营养素。基于荧光的检测系统已经成为跟踪不同物种的位置和浓度的有效方法。为了实现食品样品和生物系统中Cys的有效监测,构建了一种新型的脂质滴(LD)靶向荧光探针(NIT-Cys),用于Cys的启动检测,以大斯托克斯位移(142nm)为特征,响应时间短(<8分钟),和低Cys检测极限(39nM)。此外,NIT-Cys探针不仅已成功用于量化所选食物样品中的Cys含量,而且还可以使对乙酰氨基酚(APAP)诱导的药物诱导的肝损伤细胞中内源性Cys的可视化,斑马鱼幼虫和小鼠模型。因此,这里介绍的工作为监测Cys提供了一种有效的工具。