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Abstract：
Orexin in cerebrospinal fluid (CSF) is a neuropeptide synthesized by a cluster of neurons in the lateral hypothalamus. It mainly functions to maintain arousal, regulate feeding, and participate in reward mechanisms. Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) can detect CSF orexin. At present, RIA is widely used but is limited by various conditions, which is not conducive to its widespread development. We aimed to determine whether ELISA can replace RIA in detecting orexin in CSF. We investigated the results of 20 patients with central disorders of hypersomnolence, including 11 with narcolepsy type 1, 2 with narcolepsy type 2, 5 with idiopathic hypersomnia, and 2 with other causes of somnolence. RIA and ELISA were used to detect CSF orexin, and P values <.05 were considered to be significant. In the narcolepsy and non-narcolepsy type 1 groups, there was no correlation between the RIA and ELISA results (P > .05). In the narcolepsy type 1 group, the ELISA and RIA results were significantly different (P < .05), but this was not observed in the non-narcolepsy type 1 group (P > .05). The accuracy of ELISA to detect CSF orexin was lower than that of RIA (P < .05). ELISA cannot replace RIA in the measurement of CSF orexin, and RIA is recommended as the first choice when narcolepsy is suspected.
摘要：
脑脊液（CSF）中的Orexin是由下丘脑外侧神经元簇合成的神经肽。它的主要功能是保持唤醒，调节喂养，并参与奖励机制。放射免疫法（RIA）和酶联免疫吸附法（ELISA）可以检测CSF食欲素。目前,RIA被广泛使用，但受到各种条件的限制，不利于其广泛发展。我们旨在确定ELISA是否可以替代RIA检测CSF中的食欲素。我们调查了20例中枢嗜睡症患者的结果，包括11例发作性睡病1型，2例发作性睡病2型，5例特发性睡眠过度，和2与其他原因的嗜睡。采用RIA和ELISA检测脑脊液食欲素,P值<0.05被认为是显著的。在发作性睡病和非发作性睡病1型组中，RIA与ELISA结果无相关性(P>.05)。在发作性睡病1型组中，ELISA和RIA结果差异有统计学意义（P<0.05）。但在非发作性睡病1型组中未观察到这种情况（P>.05）。ELISA检测CSF食欲素的准确性低于RIA（P<0.05）。在CSF食欲素的测量中，ELISA不能代替RIA，当怀疑发作性睡病时，建议首选RIA。
