Abstract：
OBJECTIVE: This study evaluated the impact of the partial exposition of the nonabsorbable membrane (dPTFE) on microbial colonization during bone healing.
METHODS: Patients indicated for tooth extraction were randomized to dPTFE group (n = 22) - tooth extraction and alveolar ridge preservation (ARP) using an intentionally exposed dPTFE membrane and USH group (n = 22) - tooth extraction and unassisted socket healing. Biofilm samples were collected at the barrier in the dPTFE and on the natural healing site in the USH after 3 and 28 days. Samples from the inner surface of the dPTFE barrier were also collected (n = 13). The microbiome was evaluated using the Illumina MiSeq system.
RESULTS: Beta diversity was different from 3 to 28 days in both groups, and at 28 days, different microbial communities were identified between therapies. The dPTFE was characterized by a higher prevalence and abundance of gram-negative and anaerobic species than USH. Furthermore, the inner surface of the dPTFE membrane was colonized by a different community than the one observed on the outer surface.
CONCLUSIONS: Intentionally exposed dPTFE membrane modulates microbial colonization in the ARP site, creating a more homogeneous and anaerobic community on the inner and outer surfaces of the membrane.
CONCLUSIONS: DPTFE promoted faster biofilm colonization and enrichment of gram-negative and anaerobes close to the regenerated site in the membrane\'s inner and outer surfaces. dPTFE membrane can be used exposed to the oral site, but approaches for biofilm control should still be considered. The study was retrospectively registered at Clinicaltrials.gov (NCT04329351).
METHODS: Patients indicated for tooth extraction were randomized to dPTFE group (n = 22) - tooth extraction and alveolar ridge preservation (ARP) using an intentionally exposed dPTFE membrane and USH group (n = 22) - tooth extraction and unassisted socket healing. Biofilm samples were collected at the barrier in the dPTFE and on the natural healing site in the USH after 3 and 28 days. Samples from the inner surface of the dPTFE barrier were also collected (n = 13). The microbiome was evaluated using the Illumina MiSeq system.
RESULTS: Beta diversity was different from 3 to 28 days in both groups, and at 28 days, different microbial communities were identified between therapies. The dPTFE was characterized by a higher prevalence and abundance of gram-negative and anaerobic species than USH. Furthermore, the inner surface of the dPTFE membrane was colonized by a different community than the one observed on the outer surface.
CONCLUSIONS: Intentionally exposed dPTFE membrane modulates microbial colonization in the ARP site, creating a more homogeneous and anaerobic community on the inner and outer surfaces of the membrane.
CONCLUSIONS: DPTFE promoted faster biofilm colonization and enrichment of gram-negative and anaerobes close to the regenerated site in the membrane\'s inner and outer surfaces. dPTFE membrane can be used exposed to the oral site, but approaches for biofilm control should still be considered. The study was retrospectively registered at Clinicaltrials.gov (NCT04329351).
摘要：
目的：本研究评估了不可吸收膜（dPTFE）部分暴露对骨愈合过程中微生物定植的影响。
方法：需要拔牙的患者被随机分为dPTFE组（n=22）-使用有意暴露的dPTFE膜拔牙和牙槽脊保留（ARP）和USH组（n=22）-拔牙和无辅助牙槽愈合。在3天和28天后,在dPTFE中的屏障处和USH中的自然愈合位点上收集生物膜样品。还收集来自dPTFE屏障的内表面的样品(n=13)。使用IlluminaMiSeq系统评估微生物组。
结果：两组的Beta多样性在3到28天之间有所不同，在28天，不同的微生物群落在治疗之间进行了鉴定。与USH相比，dPTFE的特征是革兰氏阴性和厌氧物种的患病率和丰度更高。此外，dPTFE膜的内表面由与在外表面上观察到的不同的群落定殖。
结论：有意暴露的dPTFE膜可调节ARP部位的微生物定植，在膜的内表面和外表面上创造一个更均匀和厌氧的群落。
结论：DPTFE促进了生物膜更快的定植，并在膜内外表面的再生位点附近富集了革兰氏阴性和厌氧菌。dPTFE膜可用于暴露于口腔部位，但仍应考虑控制生物膜的方法。该研究在Clinicaltrials.gov（NCT04329351）进行了回顾性注册。
方法：需要拔牙的患者被随机分为dPTFE组（n=22）-使用有意暴露的dPTFE膜拔牙和牙槽脊保留（ARP）和USH组（n=22）-拔牙和无辅助牙槽愈合。在3天和28天后,在dPTFE中的屏障处和USH中的自然愈合位点上收集生物膜样品。还收集来自dPTFE屏障的内表面的样品(n=13)。使用IlluminaMiSeq系统评估微生物组。
结果：两组的Beta多样性在3到28天之间有所不同，在28天，不同的微生物群落在治疗之间进行了鉴定。与USH相比，dPTFE的特征是革兰氏阴性和厌氧物种的患病率和丰度更高。此外，dPTFE膜的内表面由与在外表面上观察到的不同的群落定殖。
结论：有意暴露的dPTFE膜可调节ARP部位的微生物定植，在膜的内表面和外表面上创造一个更均匀和厌氧的群落。
结论：DPTFE促进了生物膜更快的定植，并在膜内外表面的再生位点附近富集了革兰氏阴性和厌氧菌。dPTFE膜可用于暴露于口腔部位，但仍应考虑控制生物膜的方法。该研究在Clinicaltrials.gov（NCT04329351）进行了回顾性注册。
