Abstract：
BACKGROUND: Interspecific hybrids of rohu (Labeo rohita) and catla (Labeo catla) are common, especially in India due to constrained breeding. These hybrids must segregate from their wild parents as part of conservational strategies. This study intended to screen the hybrids from wild rohu and catla parents using both morphometric and molecular approaches.
RESULTS: The carp samples were collected from Jharkhand and West Bengal, India. The correlation and regression analysis of morphometric features are considered superficial but could be protracted statistically by clustering analysis and further consolidated by nucleotide variations of one mitochondrial and one nuclear gene to differentiate hybrids from their parents. Out of 21 morphometric features, 6 were used for clustering analysis that exhibited discrete separation among rohu, catla, and their hybrids when the data points were plotted in a low-dimensional 2-D plane using the first 2 principal components. Out of 40 selected single nucleotide polymorphism (SNP) positions of the COX1 gene, hybrid showed 100% similarity with catla. Concerning SNP similarity of the 18S rRNA nuclear gene, the hybrid showed 100% similarity with rohu but not with catla; exhibiting its probable parental inheritance.
CONCLUSIONS: Along with morphometric analysis, the SNP comparison study together points towards strong evidence of interspecific hybridization between rohu and catla, as these hybrids share both morphological and molecular differences with either parent. However, this study will help screen the hybrids from their wild parents, as a strategy for conservational management.
RESULTS: The carp samples were collected from Jharkhand and West Bengal, India. The correlation and regression analysis of morphometric features are considered superficial but could be protracted statistically by clustering analysis and further consolidated by nucleotide variations of one mitochondrial and one nuclear gene to differentiate hybrids from their parents. Out of 21 morphometric features, 6 were used for clustering analysis that exhibited discrete separation among rohu, catla, and their hybrids when the data points were plotted in a low-dimensional 2-D plane using the first 2 principal components. Out of 40 selected single nucleotide polymorphism (SNP) positions of the COX1 gene, hybrid showed 100% similarity with catla. Concerning SNP similarity of the 18S rRNA nuclear gene, the hybrid showed 100% similarity with rohu but not with catla; exhibiting its probable parental inheritance.
CONCLUSIONS: Along with morphometric analysis, the SNP comparison study together points towards strong evidence of interspecific hybridization between rohu and catla, as these hybrids share both morphological and molecular differences with either parent. However, this study will help screen the hybrids from their wild parents, as a strategy for conservational management.
摘要：
背景：罗胡（Labeorohita）和catla（Labeocatla）的种间杂种很常见，尤其是在印度，由于繁殖受限。作为保护策略的一部分，这些杂种必须与野生父母隔离。这项研究旨在使用形态计量学和分子方法从野生rohu和catla亲本中筛选杂种。
结果：鲤鱼样本是从贾坎德邦和西孟加拉邦收集的，印度。形态特征的相关性和回归分析被认为是肤浅的，但可以通过聚类分析在统计学上延长，并通过一个线粒体和一个核基因的核苷酸变异进一步巩固，以区分杂种与亲本。在21个形态特征中，6个用于聚类分析，显示罗湖之间的离散分离，Catla,当使用前2个主成分在低维2-D平面中绘制数据点时，它们的混合。在COX1基因的40个选定的单核苷酸多态性（SNP）位置中，杂种与Catla表现出100％的相似性。关于18SrRNA核基因的SNP相似性，杂种与rohu表现出100％的相似性，但与catla没有；表现出可能的亲本遗传。
结论：随着形态计量学分析，SNP比较研究共同指出了罗胡和卡特拉之间种间杂交的有力证据，因为这些杂种与任一亲本共享形态和分子差异。然而,这项研究将有助于从野生父母中筛选杂种，作为保守管理的战略。
结果：鲤鱼样本是从贾坎德邦和西孟加拉邦收集的，印度。形态特征的相关性和回归分析被认为是肤浅的，但可以通过聚类分析在统计学上延长，并通过一个线粒体和一个核基因的核苷酸变异进一步巩固，以区分杂种与亲本。在21个形态特征中，6个用于聚类分析，显示罗湖之间的离散分离，Catla,当使用前2个主成分在低维2-D平面中绘制数据点时，它们的混合。在COX1基因的40个选定的单核苷酸多态性（SNP）位置中，杂种与Catla表现出100％的相似性。关于18SrRNA核基因的SNP相似性，杂种与rohu表现出100％的相似性，但与catla没有；表现出可能的亲本遗传。
结论：随着形态计量学分析，SNP比较研究共同指出了罗胡和卡特拉之间种间杂交的有力证据，因为这些杂种与任一亲本共享形态和分子差异。然而,这项研究将有助于从野生父母中筛选杂种，作为保守管理的战略。
