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Abstract：
This work aimed to design a synthetic salt-inducible promoter using a cis-engineering approach. The designed promoter (PS) comprises a minimal promoter sequence for basal-level expression and upstream cis-regulatory elements (CREs) from promoters of salinity-stress-induced genes. The copy number, spacer lengths, and locations of CREs were manually determined based on their occurrence within native promoters. The initial activity profile of the synthesized PS promoter in transiently transformed N. tabacum leaves shows a seven-fold, five-fold, and four-fold increase in reporter GUS activity under salt, drought, and abscisic acid stress, respectively, at the 24-h interval, compared to the constitutive CaMV35S promoter. Analysis of gus expression in stable Arabidopsis transformants showed that the PS promoter induces over a two-fold increase in expression under drought or abscisic acid stress and a five-fold increase under salt stress at 24- and 48-h intervals, compared to the CaMV35S promoter. The promoter PS exhibits higher and more sustained activity under salt, drought, and abscisic acid stress compared to the constitutive CaMV35S.
摘要：
这项工作旨在使用顺式工程方法设计合成盐诱导型启动子。设计的启动子(PS)包含用于基础水平表达的最小启动子序列和来自盐度胁迫诱导基因的启动子的上游顺式调节元件(CREs)。副本编号,垫片长度,和CRE的位置基于它们在天然启动子内的出现而手动确定。在瞬时转化的烟草叶中合成的PS启动子的初始活性曲线显示了七倍，五倍，在盐下，记者GUS活性增加了四倍，干旱,和脱落酸胁迫，分别,在24小时间隔，与组成型CaMV35S启动子相比。对稳定的拟南芥转化体中gus表达的分析表明，PS启动子在干旱或脱落酸胁迫下以24小时和48小时的间隔诱导表达增加了两倍，在盐胁迫下增加了五倍。与CaMV35S启动子相比。启动子PS在盐下表现出更高和更持续的活性，干旱,与本构CaMV35S相比，脱落酸胁迫。
