PDF(Pubmed)
Abstract：
Antimicrobial resistance (AMR) is a major threat to global public health, and antibiotic resistance genes (ARGs) are widely distributed across humans, animals, and environment. Farming environments are emerging as a key research area for ARGs and antibiotic resistant bacteria (ARB). While the skin is an important reservoir of ARGs and ARB, transmission mechanisms between farming environments and human skin remain unclear. Previous studies confirmed that swine farm environmental exposures alter skin microbiome, but the timeline of these changes is ill defined. To improve understanding of these changes and to determine the specific time, we designed a cohort study of swine farm workers and students through collected skin and environmental samples to explore the impact of daily occupational exposure in swine farm on human skin microbiome. Results indicated that exposure to livestock-associated environments where microorganisms are richer than school environment can reshape the human skin microbiome and antibiotic resistome. Exposure of 5 h was sufficient to modify the microbiome and ARG structure in workers\' skin by enriching microorganisms and ARGs. These changes were preserved once formed. Further analysis indicated that ARGs carried by host microorganisms may transfer between the environment with workers\' skin and have the potential to expand to the general population using farm workers as an ARG vector. These results raised concerns about potential transmission of ARGs to the broader community. Therefore, it is necessary to take corresponding intervention measures in the production process to reduce the possibility of ARGs and ARB transmission.
摘要：
抗菌素耐药性(AMR)是全球公共卫生的主要威胁,抗生素抗性基因(ARGs)在人类中广泛分布，动物,和环境。农业环境正在成为ARGs和抗生素抗性细菌（ARB）的关键研究领域。虽然皮肤是ARGs和ARB的重要储库，农业环境和人类皮肤之间的传播机制仍不清楚。先前的研究证实，猪场环境暴露会改变皮肤微生物组，但是这些变化的时间表并不明确。为了提高对这些变化的理解并确定具体时间，我们设计了一项针对猪场工人和学生的队列研究，通过收集皮肤和环境样本，探讨猪场每日职业暴露对人体皮肤微生物组的影响。结果表明，暴露于与牲畜相关的环境中，微生物比学校环境更丰富，可以重塑人类皮肤微生物组和抗生素抗性组。暴露5小时足以通过富集微生物和ARG来改变工人皮肤中的微生物组和ARG结构。这些变化一旦形成就被保留下来。进一步的分析表明，宿主微生物携带的ARG可能在环境与工人皮肤之间转移，并有可能使用农场工人作为ARG载体扩展到普通人群。这些结果引起了人们对ARGs向更广泛社区的潜在传播的担忧。因此,有必要在生产过程中采取相应的干预措施，以减少ARGs和ARB传播的可能性。
