PDF(Pubmed)
Abstract：
BACKGROUND: Terpenes are important components of plant aromas, and terpene synthases (TPSs) are the key enzymes driving terpene diversification. In this study, we characterized the volatile terpenes in five different Chrysanthemum nankingense tissues. In addition, genome-wide identification and expression analysis of TPS genes was conducted utilizing an improved chromosome-scale genome assembly and tissue-specific transcriptomes. The biochemical functions of three representative TPSs were also investigated.
RESULTS: We identified tissue-specific volatile organic compound (VOC) and volatile terpene profiles. The improved Chrysanthemum nankingense genome assembly was high-quality, including a larger assembled size (3.26 Gb) and a better contig N50 length (3.18 Mb) compared to the old version. A total of 140 CnTPS genes were identified, with the majority representing the TPS-a and TPS-b subfamilies. The chromosomal distribution of these TPS genes was uneven, and 26 genes were included in biosynthetic gene clusters. Closely-related Chrysanthemum taxa were also found to contain diverse TPS genes, and the expression profiles of most CnTPSs were tissue-specific. The three investigated CnTPS enzymes exhibited versatile activities, suggesting multifunctionality.
CONCLUSIONS: We systematically characterized the structure and diversity of TPS genes across the Chrysanthemum nankingense genome, as well as the potential biochemical functions of representative genes. Our results provide a basis for future studies of terpene biosynthesis in chrysanthemums, as well as for the breeding of improved chrysanthemum varieties.
RESULTS: We identified tissue-specific volatile organic compound (VOC) and volatile terpene profiles. The improved Chrysanthemum nankingense genome assembly was high-quality, including a larger assembled size (3.26 Gb) and a better contig N50 length (3.18 Mb) compared to the old version. A total of 140 CnTPS genes were identified, with the majority representing the TPS-a and TPS-b subfamilies. The chromosomal distribution of these TPS genes was uneven, and 26 genes were included in biosynthetic gene clusters. Closely-related Chrysanthemum taxa were also found to contain diverse TPS genes, and the expression profiles of most CnTPSs were tissue-specific. The three investigated CnTPS enzymes exhibited versatile activities, suggesting multifunctionality.
CONCLUSIONS: We systematically characterized the structure and diversity of TPS genes across the Chrysanthemum nankingense genome, as well as the potential biochemical functions of representative genes. Our results provide a basis for future studies of terpene biosynthesis in chrysanthemums, as well as for the breeding of improved chrysanthemum varieties.
摘要：
背景：萜烯是植物香气的重要组成部分，和萜烯合酶（TPSs）是驱动萜烯多样化的关键酶。在这项研究中,我们表征了五种不同的菊花组织中的挥发性萜烯。此外,利用改进的染色体规模基因组组装和组织特异性转录组进行了TPS基因的全基因组鉴定和表达分析。还研究了三种代表性TPS的生化功能。
结果：我们鉴定了组织特异性挥发性有机化合物（VOC）和挥发性萜烯谱。改进后的南金花基因组组装是高质量的，与旧版本相比，包括更大的组装尺寸（3.26Gb）和更好的重叠群N50长度（3.18Mb）。共鉴定出140个CnTPS基因,大多数代表TPS-a和TPS-b亚科。这些TPS基因的染色体分布不均,生物合成基因簇中包含26个基因。还发现密切相关的菊花类群含有不同的TPS基因，大多数CnTSs的表达谱是组织特异性的。三种研究的CnTPS酶表现出多种活性，建议多功能性。
结论：我们系统地表征了南金花菊花基因组中TPS基因的结构和多样性，以及代表性基因的潜在生化功能。我们的研究结果为今后菊花中萜生物合成的研究提供了依据。以及用于菊花品种的选育。
结果：我们鉴定了组织特异性挥发性有机化合物（VOC）和挥发性萜烯谱。改进后的南金花基因组组装是高质量的，与旧版本相比，包括更大的组装尺寸（3.26Gb）和更好的重叠群N50长度（3.18Mb）。共鉴定出140个CnTPS基因,大多数代表TPS-a和TPS-b亚科。这些TPS基因的染色体分布不均,生物合成基因簇中包含26个基因。还发现密切相关的菊花类群含有不同的TPS基因，大多数CnTSs的表达谱是组织特异性的。三种研究的CnTPS酶表现出多种活性，建议多功能性。
结论：我们系统地表征了南金花菊花基因组中TPS基因的结构和多样性，以及代表性基因的潜在生化功能。我们的研究结果为今后菊花中萜生物合成的研究提供了依据。以及用于菊花品种的选育。
