Abstract：
JHBp2 is a peptide purified from Jinhua ham broth with antibacterial activity against Salmonella typhimurium. Untargeted metabolomics and label-free quantitative proteomics were used to analyze metabolic and protein expression changes in S. typhimurium after JHBp2 treatment. Cell wall and membrane damage results indicate that JHBp2 has membrane-disruptive properties, causing leakage of intracellular nucleic acids and proteins. Metabolomics revealed 516 differentially expressed metabolites, involving cofactor biosynthesis, purine metabolism, ABC transporters, glutathione metabolism, pyrimidine metabolism, etc. Proteomics detected 735 differentially expressed proteins, involving pyruvate metabolism, amino acid biosynthesis, purine metabolism, carbon metabolism, glycolysis/gluconeogenesis, etc. RT-qPCR and proteomics results showed a positive correlation, and molecular docking demonstrated stable binding of JHBp2 to some differentially expressed proteins. In summary, JHBp2 could disrupt the S. typhimurium cell wall and membrane structure, interfere with synthesis of membrane-related proteins, trigger intracellular substance leak, and reduce levels of enzymes and metabolites involved in energy metabolism, amino acid anabolism, and nucleotide anabolism.
摘要：
JHBp2是从金华火腿肉汤中纯化的肽，对鼠伤寒沙门氏菌具有抗菌活性。非靶向代谢组学和无标记定量蛋白质组学用于分析JHBp2治疗后鼠伤寒沙门氏菌的代谢和蛋白质表达变化。细胞壁和膜损伤结果表明，JHBp2具有膜破坏特性，导致细胞内核酸和蛋白质的泄漏。代谢组学揭示了516种差异表达的代谢物，涉及辅因子生物合成，嘌呤代谢,ABC运输商，谷胱甘肽代谢，嘧啶代谢，等。蛋白质组学检测到735种差异表达蛋白，涉及丙酮酸代谢，氨基酸生物合成，嘌呤代谢,碳代谢，糖酵解/糖异生，等。RT-qPCR与蛋白质组学结果呈正相关,和分子对接证明了JHBp2与一些差异表达蛋白的稳定结合。总之,JHBp2可以破坏鼠伤寒沙门氏菌细胞壁和膜结构，干扰膜相关蛋白的合成，引发细胞内物质泄漏，降低与能量代谢有关的酶和代谢物的水平，氨基酸合成代谢，和核苷酸合成代谢。
