PDF(Pubmed)
Abstract：
Clostridium acetobutylicum is a solventogenic, anaerobic, gram-positive bacterium that is commonly considered the model organism for studying acetone-butanol-ethanol fermentation. The need to produce these chemicals sustainably and with a minimal impact on the environment has revived the interest in research on this bacterium. The recent development of efficient genetic tools allows to better understand the physiology of this micro-organism, aiming at improving its fermentation capacities. Knowledge about gene essentiality would guide the future genetic editing strategies and support the understanding of crucial cellular functions in this bacterium. In this work, we applied a transposon insertion site sequencing method to generate large mutant libraries containing millions of independent mutants that allowed us to identify a core group of 418 essential genes needed for in vitro development. Future research on this significant biocatalyst will be guided by the data provided in this work, which will serve as a valuable resource for the community.
OBJECTIVE: Clostridium acetobutylicum is a leading candidate to synthesize valuable compounds like three and four carbons alcohols. Its ability to convert carbohydrates into a mixture of acetone, butanol, and ethanol as well as other chemicals of interest upon genetic engineering makes it an advantageous organism for the valorization of lignocellulose-derived sugar mixtures. Since, genetic optimization depends on the fundamental insights supplied by accurate gene function assignment, gene essentiality analysis is of great interest as it can shed light on the function of many genes whose functions are still to be confirmed. The data obtained in this study will be of great value for the research community aiming to develop C. acetobutylicum as a platform organism for the production of chemicals of interest.
OBJECTIVE: Clostridium acetobutylicum is a leading candidate to synthesize valuable compounds like three and four carbons alcohols. Its ability to convert carbohydrates into a mixture of acetone, butanol, and ethanol as well as other chemicals of interest upon genetic engineering makes it an advantageous organism for the valorization of lignocellulose-derived sugar mixtures. Since, genetic optimization depends on the fundamental insights supplied by accurate gene function assignment, gene essentiality analysis is of great interest as it can shed light on the function of many genes whose functions are still to be confirmed. The data obtained in this study will be of great value for the research community aiming to develop C. acetobutylicum as a platform organism for the production of chemicals of interest.
摘要：
丙酮丁醇梭菌是一种溶剂，厌氧,革兰氏阳性细菌，通常被认为是研究丙酮-丁醇-乙醇发酵的模型生物。可持续生产这些化学物质并对环境产生最小影响的需求重新引起了对这种细菌研究的兴趣。最近开发的高效遗传工具可以更好地了解这种微生物的生理学，旨在提高其发酵能力。关于基因必要性的知识将指导未来的基因编辑策略，并支持对这种细菌关键细胞功能的理解。在这项工作中,我们应用转座子插入位点测序方法,产生了包含数百万独立突变体的大型突变体文库,使我们能够鉴定出体外发育所需的418个必需基因的核心组.这项工作提供的数据将指导对这种重要的生物催化剂的未来研究，这将成为社区的宝贵资源。
目的：丙酮丁醇梭菌是合成有价值的化合物如三碳和四碳醇的主要候选物。它能够将碳水化合物转化为丙酮的混合物，丁醇,和乙醇以及其他在基因工程中感兴趣的化学物质使其成为用于木质纤维素衍生的糖混合物的价值化的有利生物体。因为,遗传优化取决于准确的基因功能分配提供的基本见解，基因必要性分析是非常感兴趣的，因为它可以揭示许多基因的功能，这些基因的功能仍有待证实。在这项研究中获得的数据对于旨在开发丙酮丁醇梭菌作为生产感兴趣的化学品的平台生物的研究社区将具有重要价值。
目的：丙酮丁醇梭菌是合成有价值的化合物如三碳和四碳醇的主要候选物。它能够将碳水化合物转化为丙酮的混合物，丁醇,和乙醇以及其他在基因工程中感兴趣的化学物质使其成为用于木质纤维素衍生的糖混合物的价值化的有利生物体。因为,遗传优化取决于准确的基因功能分配提供的基本见解，基因必要性分析是非常感兴趣的，因为它可以揭示许多基因的功能，这些基因的功能仍有待证实。在这项研究中获得的数据对于旨在开发丙酮丁醇梭菌作为生产感兴趣的化学品的平台生物的研究社区将具有重要价值。
