PDF(Pubmed)
Abstract：
BACKGROUND: Histone post-translational modifications (PTMs) are epigenetic marks that can be induced by environmental stress and elicit heritable patterns of gene expression. To investigate this process in an ecological context, we characterized the influence of salinity stress on histone PTMs within the gills, kidney, and testes of Mozambique tilapia (Oreochromis mossambicus). A total of 221 histone PTMs were quantified in each tissue sample and compared between freshwater-adapted fish exposed to salinity treatments that varied in intensity and duration.
RESULTS: Four salinity-responsive histone PTMs were identified in this study. When freshwater-adapted fish were exposed to seawater for two hours, the relative abundance of H1K16ub significantly increased in the gills. Long-term salinity stress elicited changes in both the gills and testes. When freshwater-adapted fish were exposed to a pulse of severe salinity stress, where salinity gradually increased from freshwater to a maximum of 82.5 g/kg, the relative abundance of H1S1ac significantly decreased in the gills. Under the same conditions, the relative abundance of both H3K14ac and H3K18ub decreased significantly in the testes of Mozambique tilapia.
CONCLUSIONS: This study demonstrates that salinity stress can alter histone PTMs in the gills and gonads of Mozambique tilapia, which, respectively, signify a potential for histone PTMs to be involved in salinity acclimation and adaptation in euryhaline fishes. These results thereby add to a growing body of evidence that epigenetic mechanisms may be involved in such processes.
RESULTS: Four salinity-responsive histone PTMs were identified in this study. When freshwater-adapted fish were exposed to seawater for two hours, the relative abundance of H1K16ub significantly increased in the gills. Long-term salinity stress elicited changes in both the gills and testes. When freshwater-adapted fish were exposed to a pulse of severe salinity stress, where salinity gradually increased from freshwater to a maximum of 82.5 g/kg, the relative abundance of H1S1ac significantly decreased in the gills. Under the same conditions, the relative abundance of both H3K14ac and H3K18ub decreased significantly in the testes of Mozambique tilapia.
CONCLUSIONS: This study demonstrates that salinity stress can alter histone PTMs in the gills and gonads of Mozambique tilapia, which, respectively, signify a potential for histone PTMs to be involved in salinity acclimation and adaptation in euryhaline fishes. These results thereby add to a growing body of evidence that epigenetic mechanisms may be involved in such processes.
摘要：
背景：组蛋白翻译后修饰（PTM）是表观遗传标记，可由环境胁迫诱导并引发基因表达的可遗传模式。为了在生态背景下研究这个过程，我们表征了盐度胁迫对g内组蛋白PTM的影响，肾,和莫桑比克罗非鱼（Oreochromismosambicus）的睾丸。在每个组织样品中总共定量了221种组蛋白PTM，并比较了暴露于不同强度和持续时间的盐度处理的淡水适应鱼。
结果：本研究中确定了4种盐度响应组蛋白PTM。当适应淡水的鱼暴露在海水中两个小时时，H1K16ub的相对丰度显著增加。长期的盐度胁迫会引起g和睾丸的变化。当适应淡水的鱼暴露于严重的盐度压力脉冲时，其中盐度从淡水逐渐增加到最高82.5g/kg，Hy1S1ac的相对丰度显著下降。在同样的条件下,莫桑比克罗非鱼睾丸中H3K14ac和H3K18ub的相对丰度均显着降低。
结论：这项研究表明，盐度胁迫可以改变莫桑比克罗非鱼的g和性腺中的组蛋白PTM，which,分别,表明组蛋白PTM可能参与海洋鱼类的盐度适应和适应。因此，这些结果增加了越来越多的证据，表明表观遗传机制可能参与此类过程。
结果：本研究中确定了4种盐度响应组蛋白PTM。当适应淡水的鱼暴露在海水中两个小时时，H1K16ub的相对丰度显著增加。长期的盐度胁迫会引起g和睾丸的变化。当适应淡水的鱼暴露于严重的盐度压力脉冲时，其中盐度从淡水逐渐增加到最高82.5g/kg，Hy1S1ac的相对丰度显著下降。在同样的条件下,莫桑比克罗非鱼睾丸中H3K14ac和H3K18ub的相对丰度均显着降低。
结论：这项研究表明，盐度胁迫可以改变莫桑比克罗非鱼的g和性腺中的组蛋白PTM，which,分别,表明组蛋白PTM可能参与海洋鱼类的盐度适应和适应。因此，这些结果增加了越来越多的证据，表明表观遗传机制可能参与此类过程。
