PDF(Pubmed)
Abstract：
The cilium, a pivotal organelle crucial for cell signaling and proper cell function, relies on meticulous macromolecular transport from the cytoplasm for its formation and maintenance. While the intraflagellar transport (IFT) pathway has traditionally been the focus of extensive study concerning ciliogenesis and ciliary maintenance, recent research highlights a complementary and alternative mechanism-vesicle-assisted transport (VAT) in cytoplasm to cilium trafficking. Despite its potential significance, the VAT pathway remains largely uncharacterized. This review explores recent studies providing evidence for the dynamics of vesicle-related diffusion and transport within the live primary cilium, employing high-speed super-resolution light microscopy. Additionally, we analyze the spatial distribution of vesicles in the cilium, mainly relying on electron microscopy data. By scrutinizing the VAT pathways that facilitate cargo transport into the cilium, with a specific emphasis on recent advancements and imaging data, our objective is to synthesize a comprehensive model of ciliary transport through the integration of IFT-VAT mechanisms.
摘要：
纤毛,对细胞信号传导和适当的细胞功能至关重要的细胞器，依赖于从细胞质中细致的大分子运输来形成和维持。虽然在传统上，滑膜内运输（IFT）途径一直是有关纤毛发生和纤毛维持的广泛研究的重点，最近的研究强调了一种互补和替代的机制-细胞质中的囊泡辅助运输（VAT）到纤毛运输。尽管它具有潜在的意义，增值税途径在很大程度上仍然没有特征。这篇综述探讨了最近的研究，为活的初级纤毛内囊泡相关的扩散和运输的动力学提供了证据。采用高速超分辨率光学显微镜。此外,我们分析了纤毛中囊泡的空间分布，主要依靠电子显微镜数据。通过仔细检查促进货物运输进入纤毛的增值税途径，特别强调最近的进步和成像数据，我们的目标是通过整合IFT-VAT机制来综合纤毛运输的综合模型。
