PDF(Pubmed)
Abstract：
Carbapenem-resistant Acinetobacter baumannii (CRAB) is a major human pathogen and a research priority for developing new antimicrobial agents. CRAB is a causative agent of a variety of infections in different body sites. One of the manifestations is catheter-associated urinary tract infection, which exposes the bacteria to the host\'s urine, creating a particular environment. Exposure of two CRAB clinical isolates, AB5075 and AMA40, to human urine (HU) resulted in the differential expression levels of 264 and 455 genes, respectively, of which 112 were common to both strains. Genes within this group play roles in metabolic pathways such as phenylacetic acid (PAA) catabolism, the Hut system, the tricarboxylic acid (TCA) cycle, and other processes like quorum sensing and biofilm formation. These results indicate that the presence of HU induces numerous adaptive changes in gene expression of the infecting bacteria. These modifications presumably help bacteria establish and thrive in the hostile conditions in the urinary tract. These analyses advance our understanding of CRAB\'s metabolic adaptations to human fluids, as well as expanding knowledge on bacterial responses to distinct human fluids containing different concentrations of human serum albumin (HSA).
摘要：
耐碳青霉烯类鲍曼不动杆菌（CRAB）是一种主要的人类病原体，是开发新抗菌药物的研究重点。CRAB是不同身体部位的多种感染的病原体。表现之一是导尿管相关尿路感染,使细菌暴露在宿主的尿液中，创造一个特定的环境。两种CRAB临床分离株的暴露，AB5075和AMA40对人尿（HU）导致264和455个基因的差异表达水平，分别,其中112个是两种菌株共有的。该组中的基因在代谢途径中发挥作用，例如苯乙酸（PAA）分解代谢，小屋系统,三羧酸（TCA）循环，和其他过程，如群体感应和生物膜形成。这些结果表明HU的存在诱导感染细菌的基因表达的许多适应性变化。这些修饰可能有助于细菌在泌尿道的敌对条件下建立和繁殖。这些分析促进了我们对CRAB对人体液体的代谢适应的理解，以及扩大有关细菌对含有不同浓度人血清白蛋白（HSA）的不同人体液体的反应的知识。
