PDF(Pubmed)
Abstract：
The European brittle star Amphiura filiformis emits blue light, via a Renilla-like luciferase, which depends on the dietary acquisition of coelenterazine. Questions remain regarding luciferin availability across seasons and the persistence of luminous capabilities after a single boost of coelenterazine. To date, no study has explored the seasonal, long-term monitoring of these luminous capabilities or the tracking of luciferase expression in photogenic tissues. Through multidisciplinary analysis, we demonstrate that luminous capabilities evolve according to the exogenous acquisition of coelenterazine throughout adult life. Moreover, no coelenterazine storage forms are detected within the arms tissues. Luciferase expression persists throughout the seasons, and coelenterazine\'s presence in the brittle star diet is the only limiting factor for the bioluminescent reaction. No seasonal variation is observed, involving a continuous presence of prey containing coelenterazine. The ultrastructure description provides a morphological context to investigate the green autofluorescence signal attributed to coelenterazine during luciferin acquisition. Finally, histological analyses support the hypothesis of a pigmented sheath leading light to the tip of the spine. These insights improve our understanding of the bioluminescence phenomenon in this burrowing brittle star.
摘要：
欧洲脆性恒星Amphiurafiliformis发出蓝光，通过Renilla类荧光素酶,这取决于腔肠素的饮食获取。关于荧光素跨季节的可用性以及一次增强腔肠素之后发光能力的持久性，仍然存在疑问。迄今为止,没有研究探索季节性，长期监测这些发光能力或跟踪光生组织中荧光素酶的表达。通过多学科分析,我们证明，发光能力在整个成年生活中根据腔肠素的外源性获取而发展。此外，在臂组织内没有检测到腔肠素储存形式。荧光素酶的表达在整个季节持续存在，和腔肠素的存在脆性星的饮食是唯一的限制因素的生物发光反应。没有观察到季节性变化，涉及持续存在含有腔肠素的猎物。超微结构描述提供了形态学背景，以研究荧光素获取过程中归因于腔肠素的绿色自发荧光信号。最后,组织学分析支持色素鞘将光线引导到脊柱尖端的假设。这些见解提高了我们对这个挖掘脆性恒星中生物发光现象的理解。
