Abstract：
Phthalate plasticizers (PAEs) illegally used in food pose a great threat to human health. A new and efficient sensing platform for the sensitive detection of the PAE residues in biological fluids needs to be designed and developed. Here, we report a simple and reliable surface-enhanced Raman spectroscopy (SERS) active platform with extralong hot spots of Au nanobipyramids@Ag nanorods (Au NBPs@Ag NRs) for the rapid and sensitive detection of PAEs in biological fluids. To achieve high activity, Au NBPs@Ag NRs with different shell lengths were fabricated by controlling the synthesis conditions, and the corresponding SERS properties were investigated by using crystal violet (CryV) and butyl benzyl phthalate (BBP). The experimental results showed that a longer shell length correlated to greater Raman activity, which was confirmed by finite-difference time-domain (FDTD) electromagnetic simulation. More importantly, the extralong hot spots of the Au NBPs@Ag NR SERS-active substrate showed excellent homogeneity and reproducibility for the CryV probe molecules (6.21%), and the detection limit was 10-9 M for both BBP and diethylhexyl phthalate (DEHP). Furthermore, through the standard addition method, an extralong hot spots SERS substrate could achieve highly sensitive detection of BBP and DEHP in serum and tears fluids, and the detection limit was as low as 3.52 × 10-8 M and 2.82 × 10-8 M. Therefore, the Au NBPs@Ag NR substrate with an extraordinarily long surface is efficient and versatile, and can potentially be used for high-efficiency sensing analysis in complex biological fluids.
摘要：
邻苯二甲酸酯类增塑剂(PAEs)在食品中的非法使用对人类健康构成极大威胁。需要设计和开发一种用于灵敏检测生物流体中PAE残留的新型高效传感平台。这里,我们报告了一个简单可靠的表面增强拉曼光谱（SERS）活性平台，具有Au纳米金字塔@Ag纳米棒（AuNBPs@AgNRs）的热点，可快速，灵敏地检测生物流体中的PAEs。为了实现高活性，通过控制合成条件，制备了具有不同壳长度的AuNBPs@AgNRs，用结晶紫(CryV)和邻苯二甲酸丁酯苄酯(BBP)研究了相应的SERS性能。实验结果表明，更长的壳长度与更大的拉曼活性相关，时域有限差分(FDTD)电磁仿真证实了这一点。更重要的是,AuNBPs@AgNRSERS活性底物的外热点对CryV探针分子表现出优异的均匀性和再现性（6.21%），BBP和邻苯二甲酸二乙基己酯（DEHP）的检出限为10-9M。此外，通过标准添加方法，超热点SERS基底可以实现血清和泪液中BBP和DEHP的高灵敏度检测，检测限低至3.52×10-8M和2.82×10-8M。AuNBP@AgNR基材具有非常长的表面是有效和通用的，可用于复杂生物流体的高效传感分析。
