PDF(Pubmed)
Abstract：
There is an urgent need for new bioactive molecules with unique mechanisms of action and chemistry to address the issue of incorrect use of chemical fertilizers and pesticides, which hurts both the environment and the health of humans. In light of this, research was done for this work to isolate, identify, and evaluate the germination-promoting potential of various plant species\' fungal endophytes. Zea mays L. (maize) seed germination was examined using spore suspension of 75 different endophytic strains that were identified. Three promising strains were identified through screening to possess the ability mentioned above. These strains Alternaria alternate, Aspergilus flavus, and Aspergillus terreus were isolated from the stem of Tecoma stans, Delonix regia, and Ricinus communis, respectively. The ability of the three endophytic fungal strains to produce siderophore and indole acetic acid (IAA) was also examined. Compared to both Aspergillus flavus as well as Aspergillus terreus, Alternaria alternata recorded the greatest rates of IAA, according to the data that was gathered. On CAS agar versus blue media, all three strains failed to produce siderophores. Moreover, the antioxidant and antifungal potentials of extracts from these fungi were tested against different plant pathogens. The obtained results indicated the antioxidant and antifungal activities of the three fungal strains. GC-Mass studies were carried out to determine the principal components in extracts of all three strains of fungi. The three strains\' fungus extracts included both well-known and previously unidentified bioactive compounds. These results may aid in the development of novel plant growth promoters by suggesting three different fungal strains as sources of compounds that may improve seed germination. According to the study that has been given, as unexplored sources of bioactive compounds, fungal endophytes have great potential.
摘要：
迫切需要具有独特作用和化学机制的新型生物活性分子，以解决不正确使用化学肥料和农药的问题，这既伤害了环境，也伤害了人类的健康。鉴于此,这项工作进行了研究，以隔离，identify,并评估各种植物内生真菌的发芽促进潜力。使用鉴定的75种不同内生菌株的孢子悬浮液检查了玉米L.（玉米）种子萌发。通过筛选鉴定出具有上述能力的三个有希望的菌株。这些菌株交替出现，黄曲霉,土曲霉是从Tecomastans的茎中分离出来的，Delonixregia,和蓖麻，分别。还检查了三种内生真菌菌株产生铁载体和吲哚乙酸（IAA）的能力。与黄曲霉和土曲霉相比，Alternariaalternata记录了最大的IAA率，根据收集的数据。在CAS琼脂与蓝色培养基上，所有三个菌株都没有产生铁载体。此外，测试了这些真菌提取物对不同植物病原体的抗氧化和抗真菌能力。获得的结果表明了三种真菌菌株的抗氧化和抗真菌活性。进行GC-Mass研究以确定所有三种真菌菌株的提取物中的主要成分。三株真菌提取物包括众所周知的和以前未鉴定的生物活性化合物。通过提出三种不同的真菌菌株作为可能改善种子萌发的化合物的来源，这些结果可能有助于新型植物生长促进剂的开发。根据已经给出的研究，作为生物活性化合物的未开发来源，内生真菌具有巨大的潜力。
