PDF(Pubmed)
Abstract：
BACKGROUND: Human breast milk (HBM) is a contributing factor in modulating the infant\'s gut microbiota, as it contains bacteria that are directly transferred to the infant during breastfeeding. It has been shown that children of women diagnosed with gestational diabetes mellitus (GDM) have a different gut microbiota compared to children of women without GDM. Our hypothesis is therefore that women with GDM have a different HBM microbiota, which may influence the metabolic function and capacity of the child later in life. The aim of this study was to investigate whether women with GDM have a different breast milk microbiota 1-3 weeks postpartum compared to women without GDM.
METHODS: In this case-control study, a total of 45 women were included: 18 women with GDM and 27 women without GDM. A milk sample was collected from each participant 1 to 3 weeks postpartum and the bacterial composition was examined by 16 S rRNA gene sequencing targeting the V4 region.
RESULTS: High relative abundances of Streptococcus and Staphylococcus were present in samples from both women with and without GDM. No difference could be seen in either alpha diversity, beta diversity, or specific taxa between groups.
CONCLUSIONS: Our results did not support the existence of a GDM-associated breast milk microbiota at 1-3 weeks postpartum. Further research is needed to fully understand the development of the gut microbiota of infants born to mothers with GDM.
METHODS: In this case-control study, a total of 45 women were included: 18 women with GDM and 27 women without GDM. A milk sample was collected from each participant 1 to 3 weeks postpartum and the bacterial composition was examined by 16 S rRNA gene sequencing targeting the V4 region.
RESULTS: High relative abundances of Streptococcus and Staphylococcus were present in samples from both women with and without GDM. No difference could be seen in either alpha diversity, beta diversity, or specific taxa between groups.
CONCLUSIONS: Our results did not support the existence of a GDM-associated breast milk microbiota at 1-3 weeks postpartum. Further research is needed to fully understand the development of the gut microbiota of infants born to mothers with GDM.
摘要：
背景：人母乳（HBM）是调节婴儿肠道菌群的一个促成因素，因为它含有在母乳喂养期间直接转移给婴儿的细菌。已经表明，与没有GDM的妇女的孩子相比，被诊断患有妊娠糖尿病（GDM）的妇女的孩子具有不同的肠道微生物群。因此，我们的假设是GDM女性有不同的HBM微生物群，这可能会影响孩子以后的代谢功能和能力。这项研究的目的是调查与没有GDM的女性相比，GDM的女性产后1-3周是否具有不同的母乳微生物群。
方法：在本病例对照研究中，共纳入45名女性:18名GDM女性和27名无GDM女性.产后1至3周从每个参与者收集牛奶样品，并通过靶向V4区域的16SrRNA基因测序检查细菌组成。
结果：在患有和未患有GDM的女性的样本中都存在高相对丰度的链球菌和葡萄球菌。在两个阿尔法多样性中都看不到差异，β多样性,或群体之间的特定分类单元。
结论：我们的结果不支持产后1-3周存在GDM相关的母乳菌群。需要进一步的研究来充分了解GDM母亲所生的婴儿的肠道微生物群的发育。
方法：在本病例对照研究中，共纳入45名女性:18名GDM女性和27名无GDM女性.产后1至3周从每个参与者收集牛奶样品，并通过靶向V4区域的16SrRNA基因测序检查细菌组成。
结果：在患有和未患有GDM的女性的样本中都存在高相对丰度的链球菌和葡萄球菌。在两个阿尔法多样性中都看不到差异，β多样性,或群体之间的特定分类单元。
结论：我们的结果不支持产后1-3周存在GDM相关的母乳菌群。需要进一步的研究来充分了解GDM母亲所生的婴儿的肠道微生物群的发育。
