PDF(Pubmed)
Abstract：
Physella acuta is a freshwater snail native to North America. Understanding the phylogeography and genetic structure of P. acuta will help elucidate its evolution. In this study, we used mitochondrial (COI and 16S rDNA) and nuclear (ITS1) markers to identify the species and examine its genetic diversity, population structure, and demographic history of P. acuta in Thailand. Phylogenetic and network analyses of P. acuta in Thailand pertained to clade A, which exhibits a global distribution. Analysis of the genetic structure of the population revealed that the majority of pairwise comparisons showed no genetic dissimilarity. An isolation-by-distance test indicates no significant correlation between genetic and geographical distances among P. acuta populations, suggesting that gene flow is not restricted by distance. Demographic history and haplotype network analyses suggest a population expansion of P. acuta, as evidenced by the star-like structure detected in the median-joining network. Based on these results, we concluded that P. acuta in Thailand showed gene flow and recent population expansion. Our findings provide fundamental insights into the genetic variation of P. acuta in Thailand.
摘要：
Physellaacuta是原产于北美的淡水蜗牛。了解acuta的系统地理学和遗传结构将有助于阐明其进化。在这项研究中,我们使用线粒体（COI和16SrDNA）和核（ITS1）标记来鉴定物种并检查其遗传多样性，人口结构,以及泰国阿库塔疟原虫的人口历史。泰国与进化枝A有关的acuta的系统发育和网络分析，展示了全球分布。对种群遗传结构的分析表明，大多数成对比较没有遗传差异。按距离隔离检验表明，美洲青霉种群之间的遗传距离和地理距离之间没有显着相关性，表明基因流动不受距离的限制。人口统计学史和单倍型网络分析表明，阿库塔疟原虫的种群扩张，正如在中位数加入网络中检测到的星状结构所证明的那样。基于这些结果,我们得出的结论是，泰国的acuta表现出基因流动和最近的种群扩张。我们的研究结果为泰国acuta的遗传变异提供了基本见解。
