PDF(Pubmed)
Abstract：
Forward genetic screens have been a powerful tool for discovering genes involved in various biological processes in Caenorhabditis elegans. Here, we present a protocol for forward genetic screening to identify novel factors involved in a biological process in C. elegans. We describe steps for mutagenesis, screening, and backcrossing. To save time and effort, we also detail procedures for utilizing whole-genome sequencing to exclude mutants of previously characterized genes from crosses for mapping mutations. For complete details on the use and execution of this protocol, please refer to Yoshida et al.1.
摘要：
正向遗传筛选已成为发现秀丽隐杆线虫各种生物过程中涉及的基因的有力工具。这里,我们提出了一种正向遗传筛选方案，以确定在C.elegan的生物学过程中涉及的新因素。我们描述了诱变的步骤，筛选,和反向穿越。为了节省时间和精力，我们还详细介绍了利用全基因组测序从杂交中排除先前表征基因的突变体以定位突变的程序.有关此协议的使用和执行的完整详细信息，PleaserefertoYoshidaetal.1.
