Abstract：
OBJECTIVE: The aim of this study was to explore the potential to profile and distinguish varying clinical severity grades of MIH, compared to normal enamel, using proteomics.
METHODS: Liquid chromatography-mass spectrometry analyses were conducted on enamel samples of extracted teeth, from 11 children and adolescents, spanning an age range of 6-18 years. Enamel powder samples were collected from extracted, third molars (n = 3) and first permanent molars diagnosed with MIH (n = 8). The MIH tooth samples were categorized into subgroups based on clinical severity grade. The data were statistically analyzed using ANOVA and Welch\'s t test.
RESULTS: Teeth affected by MIH exhibited a diverse array of proteins, each with different functions related to dental enamel, distinguishing them from their normal enamel counterparts. The application of microdissection combined with LC-MS techniques has revealed the potential to discern unique proteomic profiles among MIH-affected teeth, characterized by varying clinical severity grades. Both analyzed MIH groups displayed consistent trends in the presentation of biological processes, including underabundance of proteins primarily associated with cell organization and biogenesis. Furthermore, proteins linked to cell death were overabundant in both MIH groups.
CONCLUSIONS: Proteomics enabled the detection and differentiation of various proteins across different clinical severity grades of MIH.
METHODS: Liquid chromatography-mass spectrometry analyses were conducted on enamel samples of extracted teeth, from 11 children and adolescents, spanning an age range of 6-18 years. Enamel powder samples were collected from extracted, third molars (n = 3) and first permanent molars diagnosed with MIH (n = 8). The MIH tooth samples were categorized into subgroups based on clinical severity grade. The data were statistically analyzed using ANOVA and Welch\'s t test.
RESULTS: Teeth affected by MIH exhibited a diverse array of proteins, each with different functions related to dental enamel, distinguishing them from their normal enamel counterparts. The application of microdissection combined with LC-MS techniques has revealed the potential to discern unique proteomic profiles among MIH-affected teeth, characterized by varying clinical severity grades. Both analyzed MIH groups displayed consistent trends in the presentation of biological processes, including underabundance of proteins primarily associated with cell organization and biogenesis. Furthermore, proteins linked to cell death were overabundant in both MIH groups.
CONCLUSIONS: Proteomics enabled the detection and differentiation of various proteins across different clinical severity grades of MIH.
摘要：
目的：这项研究的目的是探索分析和区分MIH的不同临床严重程度等级的潜力，与正常牙釉质相比，使用蛋白质组学。
方法：对拔牙的牙釉质样品进行液相色谱-质谱分析，来自11名儿童和青少年，年龄范围为6-18岁。从提取物中收集牙釉质粉末样品，诊断为MIH的第三磨牙（n=3）和第一恒磨牙（n=8）。根据临床严重程度等级将MIH牙齿样品分为亚组。数据采用方差分析和Welcht检验进行统计分析。
结果：受MIH影响的牙齿表现出多种蛋白质，每个都有不同的功能，与牙釉质有关，将它们与正常的搪瓷相区别。显微解剖与LC-MS技术相结合的应用揭示了在MIH受影响的牙齿中辨别独特蛋白质组学特征的潜力。以不同的临床严重程度等级为特征。两个分析的MIH组在生物过程的呈现方面显示出一致的趋势，包括主要与细胞组织和生物发生相关的蛋白质丰度不足。此外，与细胞死亡相关的蛋白质在两个MIH组中都过多。
结论：蛋白质组学能够检测和区分MIH不同临床严重程度等级的各种蛋白质。
方法：对拔牙的牙釉质样品进行液相色谱-质谱分析，来自11名儿童和青少年，年龄范围为6-18岁。从提取物中收集牙釉质粉末样品，诊断为MIH的第三磨牙（n=3）和第一恒磨牙（n=8）。根据临床严重程度等级将MIH牙齿样品分为亚组。数据采用方差分析和Welcht检验进行统计分析。
结果：受MIH影响的牙齿表现出多种蛋白质，每个都有不同的功能，与牙釉质有关，将它们与正常的搪瓷相区别。显微解剖与LC-MS技术相结合的应用揭示了在MIH受影响的牙齿中辨别独特蛋白质组学特征的潜力。以不同的临床严重程度等级为特征。两个分析的MIH组在生物过程的呈现方面显示出一致的趋势，包括主要与细胞组织和生物发生相关的蛋白质丰度不足。此外，与细胞死亡相关的蛋白质在两个MIH组中都过多。
结论：蛋白质组学能够检测和区分MIH不同临床严重程度等级的各种蛋白质。
