Abstract：
Sub-estrus buffaloes do not exhibit estrus signs despite being cyclic contributing to extended service periods and inter-calving intervals causing significant economic loss. The present study described the effect of synthetic prostaglandin (PGF2α) on estrus behaviour, follicular and luteal morphometry, and serum estradiol (E2) and progesterone (P4) profile in sub-estrus buffaloes during the non-breeding season. The incidence of sub-estrus was 38.4% during the non-breeding season. The sub-estrus buffaloes (n = 33) were divided into two groups, viz., Control (n = 16) and PGF2α treatment (Inj. Cloprostenol 500 μg, i.m., n = 17). Estrus induction response was significantly greater in the treatment (100 vs. 18.75%, p < .001), and a relatively greater proportion of animals conceived in the treatment group (29.41 vs. 6.25%, p = .08). The time elapsed to induction of estrus and insemination following treatment was significantly lower in the treatment group than control. A significant increment in the follicle diameter (9.72 ± 0.45 vs. 13.00 ± 0.45 mm, P < .0001) and serum estradiol (E2) concentration (66.01 ± 11.92 vs. 104.9 ± 13.21 pg/mL, p = .003) observed at the post-treatment period in the PGF2α treatment group. At the same time, CL diameter was reduced significantly at a higher regression rate in the PGF2α treated buffaloes than those of control. Of the responded buffaloes, only 30% showed high-intensity estrus attributed to the expulsion of cervico-vaginal mucus (CVM), uterine tonicity, micturition, and mounting response by a teaser bull. From this study, it can be concluded that the administration of PGF2α could induce estrus in the sub-estrus buffaloes during the non-breeding season. Behavioural changes, along with sonographic observation of POF, regressing CL, and serum E2 and P4 concentration would be useful to determine the right time of insemination in sub-estrus buffaloes during non-breeding season.
摘要：
发情期水牛尽管周期性延长了服务期和产卵间隔，但并未表现出发情期迹象，从而造成了重大的经济损失。本研究描述了合成前列腺素（PGF2α）对发情行为的影响，卵泡和黄体形态测量,在非繁殖季节，亚发水牛的血清雌二醇（E2）和孕酮（P4）谱。在非繁殖季节，发情期的发生率为38.4％。亚发情水牛(n=33)分为两组，viz.,对照(n=16)和PGF2α处理(Inj.氯前列醇500μg，i.m.,n=17）。治疗中的发情诱导反应明显更大（100vs.18.75%,p<.001),治疗组中受孕的动物比例相对较高(29.41vs.6.25%,p=.08）。治疗组治疗后诱导发情和授精的时间明显低于对照组。卵泡直径的显着增加（9.72±0.45与13.00±0.45mm,P<.0001）和血清雌二醇（E2）浓度（66.01±11.92vs.104.9±13.21pg/mL,p=.003）在PGF2α治疗组中在治疗后阶段观察到。同时,与对照组相比，PGF2α处理的水牛的CL直径以更高的消退率显着降低。在回应的水牛中，只有30％的人表现出高强度发情期，归因于宫颈阴道粘液（CVM）的排出，子宫张力,排尿,和不断增加的预告片公牛的反应。从这项研究中,可以得出结论，在非繁殖季节，PGF2α的施用可以诱导亚发水牛发情。行为改变,随着POF的超声观察，回归CL，血清E2和P4浓度将有助于确定非繁殖季节亚发水牛授精的正确时间。
