Abstract：
For the first time, a method based on dispersive solid phase microextraction (D-μSPE) using commercial metal-organic frameworks coupled to liquid chromatography-triple quadrupole tandem mass spectrometry (LC-MS/MS) has been proposed for the determination of isoflavones in soy drinks. The use of commercial sorbents simplifies the sample treatment procedure and allows their application to routine analysis. Optimization of the parameters involved in the microextraction process was carried out using a Box-Behnken experimental design. Under the optimized conditions, the limits of detection ranged between 2 and 7 μg L-1; the intra-day and inter-day precision were <10 and 20%, respectively, and the recoveries were in the range of 61-120%. No significant matrix effect was found, which allowed the use of external standard calibration method. The method was successfully applied to the determination of isoflavones in commercial soy milk samples.
摘要：
第一次,提出了一种基于分散固相微萃取（D-μSPE）的方法，该方法使用商业金属-有机骨架结合液相色谱-三重四极杆串联质谱（LC-MS/MS）来测定大豆饮料中的异黄酮。商业吸附剂的使用简化了样品处理程序，并允许将其应用于常规分析。使用Box-Behnken实验设计对微萃取过程中涉及的参数进行优化。在优化条件下,检测限范围为2至7μgL-1；日内和日间精密度<10％和20％，分别,回收率在61-120%范围内。没有发现显著的基质效应,允许使用外标校准方法。该方法已成功应用于商品豆浆样品中异黄酮的测定。
