Abstract：
UNASSIGNED: Breast cancer (BC) is the most common malignancy in women globally. Significant progress has been made in developing structural nanoparticles (NPs) and formulations for targeted smart drug delivery (SDD) of pharmaceuticals, improving the precision of tumor cell targeting in therapy.
UNASSIGNED: Magnetic hyperthermia (MHT) treatment using magneto-liposomes (MLs) has emerged as a promising adjuvant cancer therapy.
UNASSIGNED: CoFe2O4 magnetic NPs (MNPs) were conjugated with nanoliposomes to form MLs, and the anticancer drug quercetin (Que) was loaded into MLs, forming Que-MLs composites for antitumor approach. The aim was to prepare Que-MLs for DD systems (DDS) under an alternating magnetic field (AMF), termed chemotherapy/hyperthermia (chemo-HT) techniques. The encapsulation efficiency (EE), drug loading capacity (DL), and drug release (DR) of Que and Que-MLs were evaluated.
UNASSIGNED: The results confirmed successful Que-loading on the surface of MLs, with an average diameter of 38 nm and efficient encapsulation into MLs (69%). In vitro, experimental results on MCF-7 breast cells using MHT showed high cytotoxic effects of novel Que-MLs on MCF-7 cells. Various analyses, including cytotoxicity, apoptosis, cell migration, western blotting, fluorescence imaging, and cell membrane internalization, were conducted. The Acridine Orange-ethidium bromide double fluorescence test identified 35% early and 55% late apoptosis resulting from Que-MLs under the chemo-HT group. TEM results indicated MCF-7 cell membrane internalization and digestion of Que-MLs, suggesting the presence of early endosome-like vesicles on the cytoplasmic periphery.
UNASSIGNED: Que-MLs exhibited multi-modal chemo-HT effects, displaying high toxicity against MCF-7 BC cells and showing promise as a potent cytotoxic agent for BC chemotherapy.
UNASSIGNED: Magnetic hyperthermia (MHT) treatment using magneto-liposomes (MLs) has emerged as a promising adjuvant cancer therapy.
UNASSIGNED: CoFe2O4 magnetic NPs (MNPs) were conjugated with nanoliposomes to form MLs, and the anticancer drug quercetin (Que) was loaded into MLs, forming Que-MLs composites for antitumor approach. The aim was to prepare Que-MLs for DD systems (DDS) under an alternating magnetic field (AMF), termed chemotherapy/hyperthermia (chemo-HT) techniques. The encapsulation efficiency (EE), drug loading capacity (DL), and drug release (DR) of Que and Que-MLs were evaluated.
UNASSIGNED: The results confirmed successful Que-loading on the surface of MLs, with an average diameter of 38 nm and efficient encapsulation into MLs (69%). In vitro, experimental results on MCF-7 breast cells using MHT showed high cytotoxic effects of novel Que-MLs on MCF-7 cells. Various analyses, including cytotoxicity, apoptosis, cell migration, western blotting, fluorescence imaging, and cell membrane internalization, were conducted. The Acridine Orange-ethidium bromide double fluorescence test identified 35% early and 55% late apoptosis resulting from Que-MLs under the chemo-HT group. TEM results indicated MCF-7 cell membrane internalization and digestion of Que-MLs, suggesting the presence of early endosome-like vesicles on the cytoplasmic periphery.
UNASSIGNED: Que-MLs exhibited multi-modal chemo-HT effects, displaying high toxicity against MCF-7 BC cells and showing promise as a potent cytotoxic agent for BC chemotherapy.
摘要：
■乳腺癌（BC）是全球女性最常见的恶性肿瘤。在开发用于药物靶向智能药物递送（SDD）的结构纳米颗粒（NP）和制剂方面取得了重大进展，提高肿瘤细胞靶向治疗的精确性。意义：使用磁性脂质体（MLs）的磁性热疗（MHT）治疗已成为一种有前途的辅助癌症治疗方法。
■CoFe2O4磁性NPs（MNPs）与纳米脂质体缀合形成MLs，抗癌药物槲皮素（Que）被装载到MLs中，形成Que-MLs复合材料用于抗肿瘤方法。目的是在交变磁场（AMF）下为DD系统（DDS）准备Que-MLs，称为化疗/热疗（chemo-HT）技术。封装效率(EE)，载药量（DL），评估Que和Que-MLs的药物释放（DR）。
■结果证实了在MLs表面成功加载Que，平均直径为38nm，可有效包封到MLs中（69％）。体外，使用MHT对MCF-7乳腺细胞的实验结果表明，新型Que-MLs对MCF-7细胞具有高细胞毒性作用。各种分析，包括细胞毒性，凋亡，细胞迁移，西方印迹,荧光成像,和细胞膜内化，进行了。吖啶橙-溴化乙锭双重荧光测试鉴定了在化学-HT组中由Que-MLs引起的35％的早期和55％的晚期凋亡。TEM结果表明MCF-7细胞膜内化和Que-MLs的消化，提示细胞质外围存在早期内体样囊泡。
■Que-MLs表现出多模态化学HT效应，对MCF-7BC细胞表现出高毒性，并有望作为BC化疗的有效细胞毒性剂。
■CoFe2O4磁性NPs（MNPs）与纳米脂质体缀合形成MLs，抗癌药物槲皮素（Que）被装载到MLs中，形成Que-MLs复合材料用于抗肿瘤方法。目的是在交变磁场（AMF）下为DD系统（DDS）准备Que-MLs，称为化疗/热疗（chemo-HT）技术。封装效率(EE)，载药量（DL），评估Que和Que-MLs的药物释放（DR）。
■结果证实了在MLs表面成功加载Que，平均直径为38nm，可有效包封到MLs中（69％）。体外，使用MHT对MCF-7乳腺细胞的实验结果表明，新型Que-MLs对MCF-7细胞具有高细胞毒性作用。各种分析，包括细胞毒性，凋亡，细胞迁移，西方印迹,荧光成像,和细胞膜内化，进行了。吖啶橙-溴化乙锭双重荧光测试鉴定了在化学-HT组中由Que-MLs引起的35％的早期和55％的晚期凋亡。TEM结果表明MCF-7细胞膜内化和Que-MLs的消化，提示细胞质外围存在早期内体样囊泡。
■Que-MLs表现出多模态化学HT效应，对MCF-7BC细胞表现出高毒性，并有望作为BC化疗的有效细胞毒性剂。
